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タイトル: Extender for Sperm Dilution in Olive Ridley Turtle (Lepidochelys olivacea) and Hawksbill Turtle (Eretmochelys imbricata) Semen
著者: SIRINARUMITR, KAITKANOKE
PATTHONG, YAMUNA
JAIMJATURONG, PREEDAPORN
WOONWONG, YONLAYONG
PETCHSAMUT, WORAJAN
LIMPASUNTISIN, PATTARAWAN
MANAWATTHANA, SONTAYA
SIRINARUMITR, THEERAPOL
SANYATHITISEREE, PORNCHAI
KORNKAEWRAT, KORNCHAI
SUITHUNMAPINUNTA, PIYAWAN
キーワード: semen
extender
olive ridley turtle
hawksbill turtle
発行日: Feb-2010
出版者: Graduate school of Informatics, Kyoto University
誌名: Proceedings of the 5th International Symposium on SEASTAR2000 and Asian Bio-logging Science (The 9th SEASTAR2000 workshop)
開始ページ: 7
終了ページ: 10
抄録: The objective of the study was to find an extender to dilute and preserve sea turtle semen. Six adult olive ridley turtles (Lepidochelys olivacea), and 4 adult hawksbill turtles (Eretmochelys imbricata) from Phuket Marine Biological Center and Eastern Marine and Coastal Resources Research Center in Thailand had semen collected using electroejaculator. The study was repeated twice in 2007 and 2008. After collection, each semen sample was divided and preserved in 8 different extenders, which were 1) refrigeration medium test yolk buffer, 2) Tyrode medium supplemented with albumin, lactate and pyruvate, 3) Beltsville poultry semen extender, 4) 3% Sodium citrate buffer, 5) Phosphatebuffered solution, 6) EEL extender, 7) 1% bovine serum albumin, and 8) HAM F-10 and kept at 4℃ and evaluated for viability (motile sperm) at 0, 0.25, 0.5, 1, 3, 6, 12, 24 and 48 hours. The results found that for 28% olive ridley turtles and 25% hawksbill turtles that their spermatozoa diluted in extender 1, and for 14% of both sea turtles that their spermatozoa diluted in extender 2 could survive for 24 hours. However, the motility from both sea turtles semen in both extenders was decreased by 50-80 %. Most sperm died after being diluted in the last 6 extenders. By conclusion, extender 1 and 2 were suitable extenders for sea turtle semen viability, however, adding other ingredients should be considered to enhance in viability.
記述: March 5-6, 2009, Bangkok, Thailand
URI: http://hdl.handle.net/2433/107345
出現コレクション:Proceedings of the 5th International Symposium on SEASTAR2000 and Asian Bio-logging Science (The 9th SEASTAR2000 workshop)

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