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| Title: | Crystallization and preliminary X-ray analysis of alginate importer from Sphingomonas sp. A1. |
| Authors: | Maruyama, Yukie
Itoh, Takafumi
Nishitani, Yu
Mikami, Bunzo
Hashimoto, Wataru
Murata, Kousaku |
| Author's alias: | 村田, 幸作 |
| Keywords: | alginate importer
AlgQ2
Sphingomonas sp. A1 |
| Issue Date: | 1-Mar-2012 |
| Publisher: | International Union of Crystallography |
| Journal title: | Acta crystallographica. Section F, Structural biology and crystallization communications |
| Volume: | 68 |
| Issue: | Pt 3 |
| Start page: | 317 |
| End page: | 320 |
| DOI: | 10.1107/S1744309112001893 |
| Abstract: | Sphingomonas sp. A1 directly incorporates alginate polysaccharides through a `superchannel' comprising a pit on the cell surface, alginate-binding proteins in the periplasm and an ABC transporter (alginate importer) in the inner membrane. Alginate importer, consisting of four subunits, AlgM1, AlgM2 and two molecules of AlgS, was crystallized in the presence of the binding protein AlgQ2. Preliminary X-ray analysis showed that the crystal diffracted to 3.3 Å resolution and belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 72.5, b = 136.8, c = 273.3 Å, suggesting the presence of one complex in the asymmetric unit. |
| Rights: | © International Union of Crystallograph. |
| URI: | http://hdl.handle.net/2433/155093 |
| PubMed ID: | 22442232 |
| Appears in Collections: | Journal Articles |
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