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タイトル: | Efficient gene targeting by TAL effector nucleases coinjected with exonucleases in zygotes |
著者: | Mashimo, Tomoji ![]() Kaneko, Takehito ![]() Sakuma, Tetsushi ![]() ![]() ![]() Kobayashi, Junya ![]() Kunihiro, Yayoi Voigt, Birger Yamamoto, Takashi Serikawa, Tadao |
著者名の別形: | 真下, 知士 |
キーワード: | Gene targeting Transgenic organisms Genetic engineering Mutagenesis |
発行日: | 13-Feb-2013 |
出版者: | Nature Publishing Group |
誌名: | Scientific Reports |
巻: | 3 |
論文番号: | 1253 |
抄録: | TAL Effector Nucleases (TALENs) are versatile tools for targeted gene editing in various species. However, their efficiency is still insufficient, especially in mammalian embryos. Here, we showed that combined expression of Exonuclease 1 (Exo1) with engineered site-specific TALENs provided highly efficient disruption of the endogenous gene in rat fibroblast cells. A similar increased efficiency of up to ~30% with Exo1 was also observed in fertilized rat eggs, and in the production of knockout rats for the albino (Tyr) gene. These findings demonstrate TALENs with Exo1 is an easy and efficient method of generating gene knockouts using zygotes, which increases the range of gene targeting technologies available to various species. |
記述: | TALエフェクターヌクレアーゼ(TALEN)を用いた効率的な遺伝子改変ラットの作製技術. 京都大学プレスリリース. 2013-02-13. |
著作権等: | This work is licensed under a Creative Commons Attribution-NonCommercial-ShareALike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/ |
URI: | http://hdl.handle.net/2433/169765 |
DOI(出版社版): | 10.1038/srep01253 |
PubMed ID: | 23409244 |
関連リンク: | https://www.kyoto-u.ac.jp/static/ja/news_data/h/h1/news6/2012/130213_1.htm |
出現コレクション: | 学術雑誌掲載論文等 |
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