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タイトル: | ROS Are Required for Mouse Spermatogonial Stem Cell Self-Renewal |
著者: | Morimoto, Hiroko Iwata, Kazumi Ogonuki, Narumi Inoue, Kimiko Ogura, Atsuo Kanatsu-Shinohara, Mito Morimoto, Takeshi Yabe-Nishimura, Chihiro Shinohara, Takashi ![]() ![]() |
著者名の別形: | 篠原, 隆司 |
発行日: | Jun-2013 |
出版者: | Elsevier |
誌名: | Cell Stem Cell |
巻: | 12 |
号: | 6 |
開始ページ: | 774 |
終了ページ: | 786 |
抄録: | Reactive oxygen species (ROS) generation is implicated in stem cell self-renewal in several tissues but is thought to be detrimental for spermatogenesis as well as spermatogonial stem cells (SSCs). Using cultured SSCs, we show that ROS are generated via the AKT and MEK signaling pathways under conditions where the growth factors glial cell line-derived neurotrophic factor and fibroblast growth factor 2 drive SSC self-renewal and, instead, stimulate self-renewal at physiological levels. SSCs depleted of ROS stopped proliferating, but they showed enhanced self-renewal when ROS levels were increased by the addition of hydrogen peroxide, which induced the phosphorylation of stress kinases p38 mitogen-activated protein kinase (MAPK) and c-jun N-terminal kinase (JNK). Moreover, ROS depletion in vivo decreased SSC number in the testis, and NADPH oxidase 1 (Nox1)-deficient SSCs exhibited reduced self-renewal division upon serial transplantation. These results suggest that ROS generated by Nox1 play critical roles in SSC self-renewal via the activation of the p38 MAPK and JNK pathways. |
記述: | 活性酸素の精子幹細胞に対する増殖促進作用を解明. 京都大学プレスリリース. 2013-06-07. |
著作権等: | © 2013 Elsevier Inc. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/174345 |
DOI(出版社版): | 10.1016/j.stem.2013.04.001 |
PubMed ID: | 23746981 |
関連リンク: | https://www.kyoto-u.ac.jp/static/ja/news_data/h/h1/news6/2013/130607_1.htm |
出現コレクション: | 学術雑誌掲載論文等 |
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