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タイトル: | Conferring the ability to utilize inorganic polyphosphate on ATP-specific NAD kinase |
著者: | Nakamichi, Yusuke Yoshioka, Aya Kawai, Shigeyuki Murata, Kousaku |
著者名の別形: | 中道, 優介 吉岡, 彩 河井, 重幸 村田, 幸作 |
キーワード: | Molecular evolution Enzyme mechanisms Kinases Protein design |
発行日: | 11-Sep-2013 |
出版者: | Nature Publishing Group |
誌名: | Scientific reports |
巻: | 3 |
論文番号: | 2632 |
抄録: | NAD kinase (NADK) is a crucial enzyme for production of NADP(+). ATP-specific NADK prefers ATP to inorganic polyphosphate [poly(P)] as a phosphoryl donor, whereas poly(P)/ATP-NADK utilizes both ATP and poly(P), and is employed in industrial mass production of NADP(+). Poly(P)/ATP-NADKs are distributed throughout Gram-positive bacteria and Archaea, whereas ATP-specific NADKs are found in Gram-negative α- and γ-proteobacteria and eukaryotes. In this study, we succeeded in conferring the ability to utilize poly(P) on γ-proteobacterial ATP-specific NADKs through a single amino-acid substitution; the substituted amino-acid residue is therefore important in determining the phosphoryl-donor specificity of γ-proteobacterial NADKs. We also demonstrate that a poly(P)/ATP-NADK created through this method is suitable for the poly(P)-dependent mass production of NADP(+). Moreover, based on our results, we provide insight into the evolution of bacterial NADKs, in particular, how NADKs evolved from poly(P)/ATP-NADKs into ATP-specific NADKs. |
記述: | ATP特異性の獲得メカニズムの解明 : 新薬と新しい物質生産系の開発に期待. 京都大学プレスリリース. 2013-09-11. |
著作権等: | © 2013 Nature Publishing Group This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/ |
URI: | http://hdl.handle.net/2433/178764 |
DOI(出版社版): | 10.1038/srep02632 |
PubMed ID: | 24022322 |
関連リンク: | https://www.kyoto-u.ac.jp/static/ja/news_data/h/h1/news6/2013/130911_1.htm |
出現コレクション: | 学術雑誌掲載論文等 |
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