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タイトル: | Differentiation-defective phenotypes revealed by large-scale analyses of human pluripotent stem cells. |
著者: | Koyanagi-Aoi, Michiyo Ohnuki, Mari Takahashi, Kazutoshi Okita, Keisuke https://orcid.org/0000-0001-5806-1090 (unconfirmed) Noma, Hisashi Sawamura, Yuka Teramoto, Ito Narita, Megumi Sato, Yoshiko Ichisaka, Tomoko Amano, Naoki Watanabe, Akira https://orcid.org/0000-0002-4381-4229 (unconfirmed) Morizane, Asuka https://orcid.org/0000-0001-7411-1828 (unconfirmed) Yamada, Yasuhiro Sato, Tosiya https://orcid.org/0000-0001-9830-013X (unconfirmed) Takahashi, Jun Yamanaka, Shinya |
著者名の別形: | 青井, 三千代 大貫, 茉里 高橋, 和利 沖田, 圭介 野間, 久史 澤村, 由香 寺本, 一糸 成田, 恵 佐藤, 美子 一阪, 朋子 天野, 直己 渡辺, 亮 森実, 飛鳥 山田, 泰広 佐藤, 俊哉 高橋, 淳 山中, 伸弥 |
発行日: | 20-Nov-2013 |
出版者: | National Academy of Sciences |
誌名: | Proceedings of the National Academy of Sciences of the United States of America |
巻: | 110 |
号: | 51 |
開始ページ: | 20569 |
終了ページ: | 20574 |
抄録: | We examined the gene expression and DNA methylation of 49 human induced pluripotent stem cells (hiPSCs) and 10 human embryonic stem cells and found overlapped variations in gene expression and DNA methylation in the two types of human pluripotent stem cell lines. Comparisons of the in vitro neural differentiation of 40 hiPSCs and 10 human embryonic stem cells showed that seven hiPSC clones retained a significant number of undifferentiated cells even after neural differentiation culture and formed teratoma when transplanted into mouse brains. These differentiation-defective hiPSC clones were marked by higher expression levels of several genes, including those expressed from long terminal repeats of specific human endogenous retroviruses. These data demonstrated a subset of hiPSC lines that have aberrant gene expression and defective potential in neural differentiation, which need to be identified and eliminated before applications in regenerative medicine. |
記述: | 大規模解析により品質の悪い多能性幹細胞の見分け方を開発. 京都大学プレスリリース. 2013-11-19. |
著作権等: | © 2013 National Academy of Sciences This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/179528 |
DOI(出版社版): | 10.1073/pnas.1319061110 |
PubMed ID: | 24259714 |
関連リンク: | https://www.kyoto-u.ac.jp/static/ja/news_data/h/h1/news6/2013_1/131119_1.htm |
出現コレクション: | 学術雑誌掲載論文等 |
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