ダウンロード数: 236
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
srep05281.pdf | 898.53 kB | Adobe PDF | 見る/開く |
タイトル: | Tug-of-war of microtubule filaments at the boundary of a kinesin- and dynein-patterned surface. |
著者: | Ikuta, Junya Kamisetty, Nagendra K Shintaku, Hirofumi https://orcid.org/0000-0002-9972-4222 (unconfirmed) Kotera, Hidetoshi Kon, Takahide Yokokawa, Ryuji https://orcid.org/0000-0002-6306-2693 (unconfirmed) |
著者名の別形: | 生田, 純也 Kamisetty, Nagendra K 新宅, 博文 小寺, 秀俊 昆, 隆英 横川, 隆司 |
キーワード: | Molecular biophysics Nanofabrication and nanopatterning |
発行日: | 2014 |
出版者: | Nature Publishing Group |
誌名: | Scientific reports |
巻: | 4 |
論文番号: | 5281 |
抄録: | Intracellular cargo is transported by multiple motor proteins. Because of the force balance of motors with mixed polarities, cargo moves bidirectionally to achieve biological functions. Here, we propose a microtubule gliding assay for a tug-of-war study of kinesin and dynein. A boundary of the two motor groups is created by photolithographically patterning gold to selectively attach kinesin to the glass and dynein to the gold surface using a self-assembled monolayer. The relationship between the ratio of two antagonistic motor numbers and the velocity is derived from a force-velocity relationship for each motor to calculate the detachment force and motor backward velocity. Although the tug-of-war involves >100 motors, values are calculated for a single molecule and reflect the collective dynein and non-collective kinesin functions when they work as a team. This assay would be useful for detailed in vitro analysis of intracellular motility, e.g., mitosis, where a large number of motors with mixed polarities are involved. |
記述: | 「分子綱引き」をおこなうナノシステムを開発 -細胞分裂や細胞内物質輸送の仕組みを知るカギに-.京都大学プレスリリース. 2014-06-17. |
著作権等: | This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/ |
URI: | http://hdl.handle.net/2433/188352 |
DOI(出版社版): | 10.1038/srep05281 |
PubMed ID: | 24923426 |
関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2014-06-13 |
出現コレクション: | 学術雑誌掲載論文等 |
このリポジトリに保管されているアイテムはすべて著作権により保護されています。