ダウンロード数: 262
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
srep05312.pdf | 969.25 kB | Adobe PDF | 見る/開く |
タイトル: | MicroRNA-33b knock-in mice for an intron of sterol regulatory element-binding factor 1 (Srebf1) exhibit reduced HDL-C in vivo. |
著者: | Horie, Takahiro ![]() ![]() Nishino, Tomohiro ![]() Baba, Osamu ![]() ![]() Kuwabara, Yasuhide ![]() Nakao, Tetsushi Nishiga, Masataka Usami, Shunsuke Izuhara, Masayasu Nakazeki, Fumiko Ide, Yuya Koyama, Satoshi Sowa, Naoya Yahagi, Naoya Shimano, Hitoshi Nakamura, Tomoyuki Hasegawa, Koji Kume, Noriaki Yokode, Masayuki ![]() Kita, Toru Kimura, Takeshi ![]() Ono, Koh |
著者名の別形: | 堀江, 貴裕 西野, 共達 尾野, 亘 |
キーワード: | miRNAs Experimental models of disease |
発行日: | 16-Jun-2014 |
出版者: | Nature Publishing Group |
誌名: | Scientific reports |
巻: | 4 |
論文番号: | 5312 |
抄録: | MicroRNAs (miRs) are small non-protein-coding RNAs that bind to specific mRNAs and inhibit translation or promote mRNA degradation. Recent reports, including ours, indicated that miR-33a located within the intron of sterol regulatory element-binding protein (SREBP) 2 controls cholesterol homeostasis and can be a possible therapeutic target for treating atherosclerosis. Primates, but not rodents, express miR-33b from an intron of SREBF1. Therefore, humanized mice, in which a miR-33b transgene is inserted within a Srebf1 intron, are required to address its function in vivo. We successfully established miR-33b knock-in (KI) mice and found that protein levels of known miR-33a target genes, such as ABCA1, ABCG1, and SREBP-1, were reduced compared with those in wild-type mice. As a consequence, macrophages from the miR-33b KI mice had a reduced cholesterol efflux capacity via apoA-I and HDL-C. Moreover, HDL-C levels were reduced by almost 35% even in miR-33b KI hetero mice compared with the control mice. These results indicate that miR-33b may account for lower HDL-C levels in humans than those in mice and that miR-33b is possibly utilized for a feedback mechanism to regulate its host gene SREBF1. Our mice will also aid in elucidating the roles of miR-33a/b in different genetic disease models. |
記述: | マウスへのマイクロRNA-33bの導入に成功 -ヒトでのHDL-コレステロールの質の改善に期待-. 京都大学プレスリリース. 2014-06-17. |
著作権等: | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
URI: | http://hdl.handle.net/2433/188354 |
DOI(出版社版): | 10.1038/srep05312 |
PubMed ID: | 24931346 |
関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2014-06-17 |
出現コレクション: | 学術雑誌掲載論文等 |
![](/dspace/image/articlelinker.gif)
このリポジトリに保管されているアイテムはすべて著作権により保護されています。