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タイトル: | Simple knockout by electroporation of engineered endonucleases into intact rat embryos. |
著者: | Kaneko, Takehito ![]() Sakuma, Tetsushi ![]() ![]() ![]() Yamamoto, Takashi Mashimo, Tomoji |
著者名の別形: | 金子, 武人 真下, 知士 |
キーワード: | Embryology Genetic engineering |
発行日: | 1-Oct-2014 |
出版者: | Nature Publishing Group |
誌名: | Scientific reports |
巻: | 4 |
論文番号: | 6382 |
抄録: | Engineered endonucleases, such as zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) system, provide a powerful approach for genome editing in animals. However, the microinjection of endonucleases into embryos requires a high skill level, is time consuming, and may cause damage to embryos. Here, we demonstrate that the electroporation of endonuclease mRNAs into intact embryos can induce editing at targeted loci and efficiently produce knockout rats. It is noteworthy that the electroporation of ZFNs resulted in an embryonic survival rate (91%) and a genome-editing rate (73%) that were more than 2-fold higher than the corresponding rates from conventional microinjection. Electroporation technology provides a simple and effective method to produce knockout animals. |
記述: | 超簡単!遺伝子改変動物作製法の開発 -エレクトロポレーション(電気穿孔)法による哺乳類受精卵への ZFN、TALEN、CRISPR-Casの導入に成功-. 京都大学プレスリリース. 2014-10-03. |
著作権等: | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. |
URI: | http://hdl.handle.net/2433/190985 |
DOI(出版社版): | 10.1038/srep06382 |
PubMed ID: | 25269785 |
関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2014-10-03-0 |
出現コレクション: | 学術雑誌掲載論文等 |
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