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タイトル: | Functional Differences between GDNF-Dependent and FGF2-Dependent Mouse Spermatogonial Stem Cell Self-Renewal |
著者: | Takashima, Seiji Kanatsu-Shinohara, Mito Tanaka, Takashi Morimoto, Hiroko Inoue, Kimiko Ogonuki, Narumi Jijiwa, Mayumi Takahashi, Masahide Ogura, Atsuo Shinohara, Takashi ![]() ![]() |
著者名の別形: | 高島, 誠司 篠原, 隆司 |
発行日: | Feb-2015 |
出版者: | Elsevier Inc. |
誌名: | Stem Cell Reports |
巻: | 4 |
号: | 3 |
開始ページ: | 489 |
終了ページ: | 502 |
抄録: | Spermatogonial stem cells (SSCs) are required for spermatogenesis. Earlier studies showed that glial cell line-derived neurotrophic factor (GDNF) was indispensable for SSC self-renewal by binding to the GFRA1/RET receptor. Mice with mutations in these molecules showed impaired spermatogenesis, which was attributed to SSC depletion. Here we show that SSCs undergo GDNF-independent self-renewal. A small number of spermatogonia formed colonies when testis fragments from a Ret mutant mouse strain were transplanted into heterologous recipients. Moreover, fibroblast growth factor 2 (FGF2) supplementation enabled in vitro SSC expansion without GDNF. Although GDNF-mediated self-renewal signaling required both AKT and MAP2K1/2, the latter was dispensable in FGF2-mediated self-renewal. FGF2-depleted testes exhibited increased levels of GDNF and were enriched for SSCs, suggesting that the balance between FGF2 and GDNF levels influences SSC self-renewal in vivo. Our results show that SSCs exhibit at least two modes of self-renewal and suggest complexity of SSC regulation in vivo. |
記述: | 精子幹細胞の新しい自己複製様式の発見 -男性不妊症の原因解明、遺伝病の発症機序の理解に期待-. 京都大学プレスリリース. 2015-02-13. |
著作権等: | ©2015 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
URI: | http://hdl.handle.net/2433/193679 |
DOI(出版社版): | 10.1016/j.stemcr.2015.01.010 |
PubMed ID: | 25684228 |
関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2015-02-13 |
出現コレクション: | 学術雑誌掲載論文等 |
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