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タイトル: | Establishment of a Therapeutic Anti-Pan HLA-Class II Monoclonal Antibody That Directly Induces Lymphoma Cell Death via Large Pore Formation. |
著者: | Matsuoka, Shuji Ishii, Yasuyuki Nakao, Atsuhito Abe, Masaaki Ohtsuji, Naomi Momose, Shuji Jin, Hui Arase, Hisashi Sugimoto, Koichi Nakauchi, Yusuke Masutani, Hiroshi Maeda, Michiyuki Yagita, Hideo Komatsu, Norio Hino, Okio |
著者名の別形: | 増谷, 弘 |
発行日: | 30-Mar-2016 |
出版者: | Public Library of Science |
誌名: | PLOS ONE |
巻: | 11 |
号: | 3 |
論文番号: | e0150496 |
抄録: | To develop a new therapeutic monoclonal Antibody (mAb) for Hodgkin lymphoma (HL), we immunized a BALB/c mouse with live HL cell lines, alternating between two HL cell lines. After hybridization, we screened the hybridoma clones by assessing direct cytotoxicity against a HL cell line not used for immunization. We developed this strategy for establishing mAb to reduce the risk of obtaining clonotypic mAb specific for single HL cell line. A newly established mouse anti-human mAb (4713) triggered cytoskeleton-dependent, but complement- and caspase-independent, cell death in HL cell lines, Burkitt lymphoma cell lines, and advanced adult T-cell leukemia cell lines. Intravenous injection of mAb 4713 in tumor-bearing SCID mice improved survival significantly. mAb 4713 was revealed to be a mouse anti-human pan-HLA class II mAb. Treatment with this mAb induced the formation of large pores on the surface of target lymphoma cells within 30 min. This finding suggests that the cell death process induced by this anti-pan HLA-class II mAb may involve the same death signals stimulated by a cytolytic anti-pan MHC class I mAb that also induces large pore formation. This multifaceted study supports the therapeutic potential of mAb 4713 for various forms of lymphoma. |
著作権等: | © 2016 Matsuoka et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
URI: | http://hdl.handle.net/2433/216532 |
DOI(出版社版): | 10.1371/journal.pone.0150496 |
PubMed ID: | 27028595 |
出現コレクション: | 学術雑誌掲載論文等 |
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