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j.celrep.2016.11.026.pdf | 11.5 MB | Adobe PDF | 見る/開く |
タイトル: | In Vitro Derivation and Propagation of Spermatogonial Stem Cell Activity from Mouse Pluripotent Stem Cells |
著者: | Ishikura, Yukiko Yabuta, Yukihiro Ohta, Hiroshi Hayashi, Katsuhiko Nakamura, Tomonori https://orcid.org/0000-0002-0173-0780 (unconfirmed) Okamoto, Ikuhiro https://orcid.org/0000-0002-2870-8321 (unconfirmed) Yamamoto, Takuya https://orcid.org/0000-0002-0022-3947 (unconfirmed) Kurimoto, Kazuki Shirane, Kenjiro Sasaki, Hiroyuki Saitou, Mitinori |
著者名の別形: | 石藏, 友紀子 斎藤, 通紀 |
キーワード: | spermatogonial stem cells embryonic stem cells primordial germ cells testes DNA methylation epigenetic reprogramming |
発行日: | 6-Dec-2016 |
出版者: | Elsevier B.V. |
誌名: | Cell Reports |
巻: | 17 |
号: | 10 |
開始ページ: | 2789 |
終了ページ: | 2804 |
抄録: | The in vitro derivation and propagation of spermatogonial stem cells (SSCs) from pluripotent stem cells (PSCs) is a key goal in reproductive science. We show here that when aggregated with embryonic testicular somatic cells (reconstituted testes), primordial germ cell-like cells (PGCLCs) induced from mouse embryonic stem cells differentiate into spermatogonia-like cells in vitro and are expandable as cells that resemble germline stem cells (GSCs), a primary cell line with SSC activity. Remarkably, GSC-like cells (GSCLCs), but not PGCLCs, colonize adult testes and, albeit less effectively than GSCs, contribute to spermatogenesis and fertile offspring. Whole-genome analyses reveal that GSCLCs exhibit aberrant methylation at vulnerable regulatory elements, including those critical for spermatogenesis, which may restrain their spermatogenic potential. Our study establishes a strategy for the in vitro derivation of SSC activity from PSCs, which, we propose, relies on faithful epigenomic regulation. |
記述: | マウス多能性幹細胞から精子幹細胞を試験管内で誘導 : 精子形成全過程の試験管内誘導の基盤形成. 京都大学プレスリリース. 2016-12-07. |
著作権等: | © 2016 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
URI: | http://hdl.handle.net/2433/217467 |
DOI(出版社版): | 10.1016/j.celrep.2016.11.026 |
PubMed ID: | 27926879 |
関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2016-12-07-2 |
出現コレクション: | 学術雑誌掲載論文等 |
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