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j.omtm.2016.12.007.pdf | 2.56 MB | Adobe PDF | 見る/開く |
タイトル: | Simple Derivation of Spinal Motor Neurons from ESCs/iPSCs Using Sendai Virus Vectors |
著者: | Goto, Kazuya https://orcid.org/0000-0003-2235-3811 (unconfirmed) Imamura, Keiko Komatsu, Kenichi Mitani, Kohnosuke Aiba, Kazuhiro Nakatsuji, Norio Inoue, Makoto Kawata, Akihiro Yamashita, Hirofumi Takahashi, Ryosuke https://orcid.org/0000-0002-1407-9640 (unconfirmed) Inoue, Haruhisa https://orcid.org/0000-0003-4736-9537 (unconfirmed) |
著者名の別形: | 後藤, 和也 今村, 恵子 小松, 研一 井上, 治久 |
キーワード: | motor neurons Sendai virus induced pluripotent stem cells embryonic stem cells iPSC ESC differentiation direct conversion transcription factor |
発行日: | 17-Mar-2017 |
出版者: | Elsevier B.V. |
誌名: | Molecular Therapy - Methods & Clinical Development |
巻: | 4 |
開始ページ: | 115 |
終了ページ: | 125 |
抄録: | Amyotrophic lateral sclerosis (ALS) is a progressive and fatal degenerative disorder of motor neurons (MNs). Embryonic stem cells (ESCs)/induced pluripotent stem cells (iPSCs) now help us to understand the pathomechanisms of ALS via disease modeling. Various methods to differentiate ESCs/iPSCs into MNs by the addition of signaling molecules have been reported. However, classical methods require multiple steps, and newer simple methods using the transduction of transcription factors run the risk of genomic integration of the vector genes. Heterogeneity of the expression levels of the transcription factors also remains an issue. Here we describe a novel approach for differentiating human and mouse ESCs/iPSCs into MNs using a single Sendai virus vector encoding three transcription factors, LIM/homeobox protein 3, neurogenin 2, and islet-1, which are integration free. This single-vector method, generating HB9-positive cells on day 2 from human iPSCs, increases the ratio of MNs to neurons compared to the use of three separate Sendai virus vectors. In addition, the MNs derived via this method from iPSCs of ALS patients and model mice display disease phenotypes. This simple approach significantly reduces the efforts required to generate MNs, and it provides a useful tool for disease modeling. |
記述: | センダイウイルスベクターを用いてES細胞/iPS細胞から脊髄運動ニューロンを簡便に作製する技術開発. 京都大学プレスリリース. 2017-02-07. |
著作権等: | © 2017 The Authors. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
URI: | http://hdl.handle.net/2433/217986 |
DOI(出版社版): | 10.1016/j.omtm.2016.12.007 |
PubMed ID: | 28344997 |
関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2017-02-07 |
出現コレクション: | 学術雑誌掲載論文等 |
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