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Title: Hydroxyethyl cellulose matrix applied to serial crystallography
Authors: Sugahara, Michihiro
Nakane, Takanori
Masuda, Tetsuya
Suzuki, Mamoru
Inoue, Shigeyuki
Song, Changyong
Tanaka, Rie
Nakatsu, Toru
Mizohata, Eiichi
Yumoto, Fumiaki
Tono, Kensuke
Joti, Yasumasa
Kameshima, Takashi
Hatsui, Takaki
Yabashi, Makina
Nureki, Osamu
Numata, Keiji
Nango, Eriko
Iwata, So
Author's alias: 菅原, 道泰
桝田, 哲哉
鈴木, 守
登野, 健介
岩田, 想
Issue Date: 6-Apr-2017
Publisher: Springer Nature
Journal title: Scientific Reports
Volume: 7
Thesis number: 703
Abstract: Serial femtosecond crystallography (SFX) allows structures of proteins to be determined at room temperature with minimal radiation damage. A highly viscous matrix acts as a crystal carrier for serial sample loading at a low flow rate that enables the determination of the structure, while requiring consumption of less than 1 mg of the sample. However, a reliable and versatile carrier matrix for a wide variety of protein samples is still elusive. Here we introduce a hydroxyethyl cellulose-matrix carrier, to determine the structure of three proteins. The de novo structure determination of proteinase K from single-wavelength anomalous diffraction (SAD) by utilizing the anomalous signal of the praseodymium atom was demonstrated using 3, 000 diffraction images.
Description: 低ノイズ・低粘着性・低コストのタンパク質結晶輸送媒体を発見--生理条件に近いタンパク質の高分解能構造解析に期待--. 京都大学プレスリリース. 2017-04-20.
Rights: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
URI: http://hdl.handle.net/2433/220412
DOI(Published Version): 10.1038/s41598-017-00761-0
Related Link: http://www.kyoto-u.ac.jp/ja/research/research_results/2017/170406_2.html
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