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| ファイル | 記述 | サイズ | フォーマット | |
|---|---|---|---|---|
| s41598-017-00761-0.pdf | 2.07 MB | Adobe PDF | 見る/開く |
| タイトル: | Hydroxyethyl cellulose matrix applied to serial crystallography |
| 著者: | Sugahara, Michihiro Nakane, Takanori Masuda, Tetsuya   https://orcid.org/0000-0001-5857-5753 (unconfirmed)Suzuki, Mamoru Inoue, Shigeyuki Song, Changyong Tanaka, Rie https://orcid.org/0000-0003-1062-4792 (unconfirmed)Nakatsu, Toru https://orcid.org/0000-0002-9582-4023 (unconfirmed)Mizohata, Eiichi Yumoto, Fumiaki Tono, Kensuke Joti, Yasumasa Kameshima, Takashi Hatsui, Takaki Yabashi, Makina Nureki, Osamu Numata, Keiji  https://orcid.org/0000-0003-2199-7420 (unconfirmed)Nango, Eriko Iwata, So |
| 著者名の別形: | 菅原, 道泰 桝田, 哲哉 鈴木, 守 登野, 健介 岩田, 想 |
| 発行日: | 6-Apr-2017 |
| 出版者: | Springer Nature |
| 誌名: | Scientific Reports |
| 巻: | 7 |
| 論文番号: | 703 |
| 抄録: | Serial femtosecond crystallography (SFX) allows structures of proteins to be determined at room temperature with minimal radiation damage. A highly viscous matrix acts as a crystal carrier for serial sample loading at a low flow rate that enables the determination of the structure, while requiring consumption of less than 1 mg of the sample. However, a reliable and versatile carrier matrix for a wide variety of protein samples is still elusive. Here we introduce a hydroxyethyl cellulose-matrix carrier, to determine the structure of three proteins. The de novo structure determination of proteinase K from single-wavelength anomalous diffraction (SAD) by utilizing the anomalous signal of the praseodymium atom was demonstrated using 3, 000 diffraction images. |
| 記述: | 低ノイズ・低粘着性・低コストのタンパク質結晶輸送媒体を発見--生理条件に近いタンパク質の高分解能構造解析に期待--. 京都大学プレスリリース. 2017-04-20. |
| 著作権等: | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
| URI: | http://hdl.handle.net/2433/220412 |
| DOI(出版社版): | 10.1038/s41598-017-00761-0 |
| PubMed ID: | 28386083 |
| 関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2017-04-20 |
| 出現コレクション: | 学術雑誌掲載論文等 |
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