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Title:	MicroRNA-133 regulates the expression of GLUT4 by targeting KLF15 and is involved in metabolic control in cardiac myocytes
Authors:	Horie, Takahiro Ono, Koh Nishi, Hitoo Iwanaga, Yoshitaka Nagao, Kazuya Kinoshita, Minako Kuwabara, Yasuhide Takanabe, Rieko Hasegawa, Koji Kita, Toru Kimura, Takeshi
Author's alias:	尾野, 亘
Keywords:	Cardiomyocyte GLUT4 MicroRNA
Issue Date:	Nov-2009
Publisher:	Elsevier
Journal title:	Biochemical and Biophysical Research Communications
Volume:	389
Issue:	2
Start page:	315
End page:	320
DOI:	10.1016/j.bbrc.2009.08.136
Abstract:	GLUT4 shows decreased levels in failing human adult hearts. We speculated that GLUT4 expression in cardiac muscle may be fine-tuned by microRNAs. Forced expression of miR-133 decreased GLUT4 expression and reduced insulin-mediated glucose uptake in cardiomyocytes. A computational miRNA target prediction algorithm showed that KLF15 is one of the targets of miR-133. It was confirmed that over-expression of miR-133 reduced the protein level of KLF15, which reduced the level of the downstream target GLUT4. Cardiac myocytes infected with lenti-decoy, in which the 3′UTR with tandem sequences complementary to miR-133 was linked to the luciferase reporter gene, had decreased miR-133 levels and increased levels of GLUT4. The expression levels of KLF15 and GLUT4 were decreased at the left ventricular hypertrophy and congestive heart failure stage in a rat model. The present results indicated that miR-133 regulates the expression of GLUT4 by targeting KLF15 and is involved in metabolic control in cardiomyocytes.
Rights:	c 2009 Elsevier Inc. All rights reserved.
URI:	http://hdl.handle.net/2433/86176
Appears in Collections:	Journal Articles

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