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タイトル: | Non-invasive age estimation based on faecal DNA using methylation-sensitive high-resolution melting for Indo-Pacific bottlenose dolphins |
著者: | Yagi, Genfu Qi, Huiyuan Arai, Kana Kita, Yuki F. Kogi, Kazunobu Morisaka, Tadamichi Yoshioka, Motoi Inoue‐Murayama, Miho |
著者名の別形: | 八木, 原風 齐, 惠元 新井, 花奈 北, 夕紀 小木, 万布 森阪, 匡通 吉岡, 基 村山, 美穂 |
キーワード: | ageing demography DNA methylation ecology epigenetics Mikura Island |
発行日: | Feb-2024 |
出版者: | Wiley |
誌名: | Molecular Ecology Resources |
巻: | 24 |
号: | 2 |
論文番号: | e13906 |
抄録: | Age is necessary information for the study of life history of wild animals. A general method to estimate the age of odontocetes is counting dental growth layer groups (GLGs). However, this method is highly invasive as it requires the capture and handling of individuals to collect their teeth. Recently, the development of DNA-based age estimation methods has been actively studied as an alternative to such invasive methods, of which many have relied on used biopsy samples. However, if DNA-based age estimation can be developed from faecal samples, age estimation can be performed entirely non-invasively. We developed an age estimation model using the methylation rate of two gene regions, GRIA2 and CDKN2A, measured through methylation-sensitive high-resolution melting (MS-HRM) from faecal samples of wild Indo-Pacific bottlenose dolphins (Tursiops aduncus). The age of individuals was known through conducting longitudinal individual identification surveys underwater. Methylation rates were quantified from 36 samples collected from 30 individuals. Both gene regions showed a significant correlation between age and methylation rate. The age estimation model was constructed based on the methylation rates of both genes which achieved sufficient accuracy (after LOOCV: MAE = 5.08, R² = 0.33) for the ecological studies of the Indo-Pacific bottlenose dolphins, with a lifespan of 40–50 years. This is the first study to report the use of non-invasive faecal samples to estimate the age of marine mammals. |
記述: | 野生イルカのうんちから年齢を推定 --野生水生動物の糞から抽出したDNAのエピジェネティッククロックを用いた年齢推定に世界で初めて成功--. 京都大学プレスリリース. 2023-12-18. |
著作権等: | © 2023 The Authors. Molecular Ecology Resources published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
URI: | http://hdl.handle.net/2433/286603 |
DOI(出版社版): | 10.1111/1755-0998.13906 |
PubMed ID: | 38041546 |
関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2023-12-18 |
出現コレクション: | 学術雑誌掲載論文等 |
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