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タイトル: | An Artificial Liposome Compartment with Size Exclusion Molecular Transport |
著者: | Zhang, Shiwei Nakata, Eiji https://orcid.org/0000-0001-9564-6805 (unconfirmed) Lin, Peng https://orcid.org/0000-0003-2601-0787 (unconfirmed) Morii, Takashi https://orcid.org/0000-0003-3663-3267 (unconfirmed) |
著者名の別形: | 中田, 栄司 森井, 孝 |
キーワード: | compartment DNA origami liposome membrane transporter size exclusion transport |
発行日: | 24-Nov-2023 |
出版者: | Wiley |
誌名: | Chemistry – A European Journal |
巻: | 29 |
号: | 66 |
論文番号: | e202302093 |
抄録: | The cellular compartment plays an essential role in organizing the complex and diverse biochemical reactions within the cell. By mimicking the function of such cellular compartments, the challenge of constructing artificial compartments has been taken up to develop new biochemical tools for efficient material production and diagnostics. The important features required for the artificial compartment are that it isolates the interior from the external environment and is further functionalized to control the transport of target chemicals to regulate the interior concentration of both substrate and reaction products. In this study, an artificial compartment with size-selective molecular transport function was constructed by using a DNA origami-guided liposome prepared by modifying the method reported by Perrault et al. This completely isolates the liposome interior, including the DNA origami skeleton, from the external environment and allows the assembly of a defined number of molecules of interest inside and/or outside the compartment. By incorporating a bacterial membrane protein, OmpF, into the liposome, the resulting artificial compartment was shown to transport only the molecule of interest with a molecular weight below 600 Da from the external environment into the interior of the compartment. |
著作権等: | © 2023 The Authors. Chemistry - A European Journal published by Wiley-VCH GmbH This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
URI: | http://hdl.handle.net/2433/286726 |
DOI(出版社版): | 10.1002/chem.202302093 |
PubMed ID: | 37668304 |
出現コレクション: | 学術雑誌掲載論文等 |
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