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タイトル: | In vitro induction of patterned branchial arch-like aggregate from human pluripotent stem cells |
著者: | Seto, Yusuke Ogihara, Ryoma Takizawa, Kaori Eiraku, Mototsugu |
著者名の別形: | 瀬戸, 裕介 荻原, 龍馬 瀧澤, 香織 永樂, 元次 |
キーワード: | Differentiation Pluripotent stem cells Stem-cell differentiation |
発行日: | 14-Feb-2024 |
出版者: | Springer Nature |
誌名: | Nature Communications |
巻: | 15 |
論文番号: | 1351 |
抄録: | Early patterning of neural crest cells (NCCs) in the craniofacial primordium is important for subsequent development of proper craniofacial structures. However, because of the complexity of the environment of developing tissues, surveying the early specification and patterning of NCCs is difficult. In this study, we develop a simplified in vitro 3D model using human pluripotent stem cells to analyze the early stages of facial development. In this model, cranial NCC-like cells spontaneously differentiate from neural plate border-like cells into maxillary arch-like mesenchyme after a long-term culture. Upon the addition of EDN1 and BMP4, these aggregates are converted into a mandibular arch-like state. Furthermore, temporary treatment with EDN1 and BMP4 induces the formation of spatially separated domains expressing mandibular and maxillary arch markers within a single aggregate. These results suggest that this in vitro model is useful for determining the mechanisms underlying cell fate specification and patterning during early facial development. |
記述: | ヒトES細胞から咽頭弓様の構造を形成 --顔の初期発生過程の解明の為のモデルを作製--. 京都大学プレスリリース. 2024-02-15. |
著作権等: | © The Author(s) 2024 This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. |
URI: | http://hdl.handle.net/2433/287030 |
DOI(出版社版): | 10.1038/s41467-024-45285-0 |
PubMed ID: | 38355589 |
関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2024-02-15-0 |
出現コレクション: | 学術雑誌掲載論文等 |
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