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タイトル: Opto-combinatorial indexing enables high-content transcriptomics by linking cell images and transcriptomes
著者: Tsuchida, Arata
Kaneko, Taikopaul
Nishikawa, Kaori
Kawasaki, Mayu
Yokokawa, Ryuji  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-6306-2693 (unconfirmed)
Shintaku, Hirofumi  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-9972-4222 (unconfirmed)
著者名の別形: 土田, 新
金子, 泰洸ポール
横川, 隆司
新宅, 博文
発行日: 21-Apr-2024
出版者: Royal Society of Chemistry (RSC)
誌名: Lab on a Chip
巻: 24
号: 8
開始ページ: 2287
終了ページ: 2297
抄録: We introduce a simple integrated analysis method that links cellular phenotypic behaviour with single-cell RNA sequencing (scRNA-seq) by utilizing a combination of optical indices from cells and hydrogel beads. With our method, the combinations, referred to as joint colour codes, enable the link via matching the optical combinations measured by conventional epi-fluorescence microscopy with the concatenated DNA molecular barcodes created by cell-hydrogel bead pairs and sequenced by next-generation sequencing. We validated our approach by demonstrating an accurate link between the cell image and scRNA-seq with mixed species experiments, longitudinal cell tagging by electroporation and lipofection, and gene expression analysis. Furthermore, we extended our approach to multiplexed chemical transcriptomics, which enabled us to identify distinct phenotypic behaviours in HeLa cells treated with various concentrations of paclitaxel, and determine the corresponding gene regulation associated with the formation of a multipolar spindle.
著作権等: This is an accepted manuscript of the paper which has been published in final form at DOI https://doi.org/10.1039/D3LC00866E
The full-text file will be made open to the public on 20 Mar 2025 in accordance with publisher's 'Terms and Conditions for Self-Archiving'
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/287554
DOI(出版社版): 10.1039/D3LC00866E
PubMed ID: 38506394
出現コレクション:学術雑誌掲載論文等

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