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ファイル | 記述 | サイズ | フォーマット | |
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j.stemcr.2024.02.009.pdf | 5.81 MB | Adobe PDF | 見る/開く |
タイトル: | Screening of factors inducing alveolar type 1 epithelial cells using human pluripotent stem cells. |
著者: | Ohnishi, Yuko Masui, Atsushi Suezawa, Takahiro Mikawa, Ryuta Hirai, Toyohiro Hagiwara, Masatoshi Gotoh, Shimpei |
著者名の別形: | 大西, 裕子 末澤, 隆浩 三河, 隆太 平井, 豊博 萩原, 正敏 後藤, 慎平 増井, 淳 |
キーワード: | iPSC alveolar epithelial differentiation high-throughput screening YAP/TAZ AKT lung regeneration organoid |
発行日: | 9-Apr-2024 |
出版者: | Elsevier BV |
誌名: | Stem cell reports |
巻: | 19 |
号: | 4 |
開始ページ: | 529 |
終了ページ: | 544 |
抄録: | Alveolar type 2 (AT2) epithelial cells are tissue stem cells capable of differentiating into alveolar type 1 (AT1) cells for injury repair and maintenance of lung homeostasis. However, the factors involved in human AT2-to-AT1 cell differentiation are not fully understood. Here, we established SFTPCGFP and AGERmCherry-HiBiT dual-reporter induced pluripotent stem cells (iPSCs), which detected AT2-to-AT1 cell differentiation with high sensitivity and identified factors inducing AT1 cell differentiation from AT2 and their progenitor cells. We also established an "on-gel" alveolar epithelial spheroid culture suitable for medium-throughput screening. Among the 274 chemical compounds, several single compounds, including LATS-IN-1, converted AT1 cells from AT2 and their progenitor cells. Moreover, YAP/TAZ signaling activation and AKT signaling suppression synergistically recapitulated the induction of transcriptomic, morphological, and functionally mature AT1 cells. Our findings provide novel insights into human lung development and lung regenerative medicine. |
記述: | ヒトiPS細胞由来肺胞スフェロイドの"on-gel培養法"による化合物スクリーニング --I型肺胞上皮細胞の分化を促進するシグナル経路を同定--. 京都大学プレスリリース. 2024-03-29. Constructing "on-gel" alveolar organoids as a new screening platform. 京都大学プレスリリース. 2024-03-29. |
著作権等: | © 2024 The Author(s). This is an open access article under the CC BY license. |
URI: | http://hdl.handle.net/2433/287612 |
DOI(出版社版): | 10.1016/j.stemcr.2024.02.009 |
PubMed ID: | 38552636 |
関連リンク: | https://www.cira.kyoto-u.ac.jp/j/pressrelease/news/240329-000000.html https://www.cira.kyoto-u.ac.jp/e/pressrelease/news/240329-000000.html |
出現コレクション: | 学術雑誌掲載論文等 |
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