DSpace コレクション:
http://hdl.handle.net/2433/49807
2024-03-19T06:47:50ZNear Quantitative Ligation Results in Resistance of DNA Origami Against Nuclease and Cell Lysate
http://hdl.handle.net/2433/286727
タイトル: Near Quantitative Ligation Results in Resistance of DNA Origami Against Nuclease and Cell Lysate
著者: Krishnamurthy, Kirankumar; Rajendran, Arivazhagan; Nakata, Eiji; Morii, Takashi
抄録: There have been limited efforts to ligate the staple nicks in DNA origami which is crucial for their stability against thermal and mechanical treatments, and chemical and biological environments. Here, two near quantitative ligation methods are demonstrated for the native backbone linkage at the nicks in origami: i) a cosolvent dimethyl sulfoxide (DMSO)-assisted enzymatic ligation and ii) enzyme-free chemical ligation by CNBr. Both methods achieved over 90% ligation in 2D origami, only CNBr-method resulted in ≈80% ligation in 3D origami, while the enzyme-alone yielded 31–55% (2D) or 22–36% (3D) ligation. Only CNBr-method worked efficiently for 3D origami. The CNBr-mediated reaction is completed within 5 min, while DMSO-method took overnight. Ligation by these methods improved the structural stability up to 30 °C, stability during the electrophoresis and subsequent extraction, and against nuclease and cell lysate. These methods are straightforward, non-tedious, and superior in terms of cost, reaction time, and efficiency.
記述: DNA折り紙に革命を起こす --新たな応用を加速する新しい構造安定化法--. 京都大学プレスリリース. 2023-11-02.2024-01-19T00:00:00ZAn Artificial Liposome Compartment with Size Exclusion Molecular Transport
http://hdl.handle.net/2433/286726
タイトル: An Artificial Liposome Compartment with Size Exclusion Molecular Transport
著者: Zhang, Shiwei; Nakata, Eiji; Lin, Peng; Morii, Takashi
抄録: The cellular compartment plays an essential role in organizing the complex and diverse biochemical reactions within the cell. By mimicking the function of such cellular compartments, the challenge of constructing artificial compartments has been taken up to develop new biochemical tools for efficient material production and diagnostics. The important features required for the artificial compartment are that it isolates the interior from the external environment and is further functionalized to control the transport of target chemicals to regulate the interior concentration of both substrate and reaction products. In this study, an artificial compartment with size-selective molecular transport function was constructed by using a DNA origami-guided liposome prepared by modifying the method reported by Perrault et al. This completely isolates the liposome interior, including the DNA origami skeleton, from the external environment and allows the assembly of a defined number of molecules of interest inside and/or outside the compartment. By incorporating a bacterial membrane protein, OmpF, into the liposome, the resulting artificial compartment was shown to transport only the molecule of interest with a molecular weight below 600 Da from the external environment into the interior of the compartment.2023-11-24T00:00:00ZNon-coding RNA suppresses FUS aggregation caused by mechanistic shear stress on pipetting in a sequence-dependent manner
http://hdl.handle.net/2433/286725
タイトル: Non-coding RNA suppresses FUS aggregation caused by mechanistic shear stress on pipetting in a sequence-dependent manner
著者: Hamad, Nesreen; Yoneda, Ryoma; So, Masatomo; Kurokawa, Riki; Nagata, Takashi; Katahira, Masato
抄録: Fused in sarcoma/translocated in liposarcoma (FUS/TLS) is a multitasking RNA/DNA binding protein. FUS aggregation is implicated in various neurodegenerative diseases. RNA was suggested to modulate phase transition of FUS. Here, we found that FUS transforms into the amorphous aggregation state as an instant response to the shear stress caused by usual pipetting even at a low FUS concentration, 100 nM. It was revealed that non-coding RNA can suppress the transformation of FUS into aggregates. The suppressive effect of RNA on FUS aggregation is sequence-dependent. These results suggested that the non-coding RNA could be a prospective suppressor of FUS aggregation caused by mechanistic stress in cells. Our finding might pave the way for more research on the role of RNAs as aggregation inhibitors, which could facilitate the development of therapies for neurodegenerative diseases.2021-05-04T00:00:00ZProposal of a Non-Destructive Detection System for Hidden Nuclear Materials Based on a Neutron/Gamma-ray Hybrid System
http://hdl.handle.net/2433/286513
タイトル: Proposal of a Non-Destructive Detection System for Hidden Nuclear Materials Based on a Neutron/Gamma-ray Hybrid System
著者: Ohgaki, H.; Kii, T.; Masuda, K.; Omer, M.; Misawa, T.; Pyeon, C. H.; Hajima, R.; Hayakawa, T.; Shizuma, T.; Kando, M.; Daido, I.; Toyokawa, H.
抄録: A nuclear material detection system based on neutron/gamma-ray hybrid system has been proposed for the container inspection at the sea port. The neutron from the inertial electrostatic confinement fusion source will be used for a fast pre-screening process by using both of neutrons and gamma-rays. The nuclear resonance fluorescence gamma-ray induced by quasi-monochromatic gamma-ray beam from the laser Compton Backscattering will be used in isotope identification for the precise post-screening process. The combination of two different probes, neutrons and gamma-rays, can detect nuclear material hidden by any kind of shielding.2011-11-01T00:00:00Z