ダウンロード数: 348
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
zs12p573.pdf | 3.51 MB | Adobe PDF | 見る/開く |
タイトル: | Activation of Xenopus Eggs by Cynops Sperm Extract is Dependent upon Both Extra-and Intra-Cellular Ca Activities |
著者: | Iwao, Yasuhiro Kobayashi, Michiko Miki, Akiko Kubota, Hiroshi Y. Yoshimoto, Yasuaki |
著者名の別形: | 久保田, 洋 |
発行日: | Oct-1995 |
出版者: | Zoological Society of Japan |
誌名: | Zoological Science |
巻: | 12 |
号: | 5 |
開始ページ: | 573 |
終了ページ: | 581 |
抄録: | When unfertilized Xenopus eggs were treated by Cynops sperm extract in 10% Steinberg's solution (SB), egg's membranes hyperpolarized to about −37 mV and then depolarized to elicit a positive-going potential amounting to about +34 mV. The eggs underwent cortical contraction and resumption of meiosis. Activation of eggs in various external solutions indicates that the hyperpolarization is due mainly to opening of Na channels, but the positive-going potential is due to Cl channels on the egg's plasma membranes. Since the activation was inhibited by CdCl_[2], CoCl_[2], or NiCl_[2] as well as by amiloride, Ca influx through Ca channels is necessary for the activation by the sperm extract. A propagative intracellular Ca release was induced not only by Cynops sperm, but also by their sperm extract. Injection of BAPTA or heparin into the eggs completely inhibited activation, indicating that egg activation requires an intracellular Ca release dependent upon receptors for inositol 1,4,5-trisphosphate. |
著作権等: | (c) 日本動物学会 / Zoological Society of Japan |
URI: | http://hdl.handle.net/2433/108630 |
DOI(出版社版): | 10.2108/zsj.12.573 |
出現コレクション: | Zoological Science |
このリポジトリに保管されているアイテムはすべて著作権により保護されています。