泌尿器科領域に於けるピロフォスファターゼの組織化学的研究 第2報:家兎実験的腎障碍時の活性分布の変動に就いて

Abstract

In report I, I have reported on the activity of pyrophosphatase of normal rabbits by use of Maeda's method and compared with phosphomonoesterase in it. And now I tried to make out the changes of activity under a few experimental renal disturbance, that is, ligation of ureter on one side, injection of corrosive sublimate and potassium dichromate. The results obtained were as follows. Chapter I. Change of pyropho s p hatase activity in rabbit's kidney under ligation of ureter on one side. In this cha p ter I reported the change of activity in rabbit's kidney under ligation of uretar on one side. (1) The k i d ney become hydronephrosis and hydronephrotic atrophy and shall divided 3 stadium. Reaction of glomerulus and tubule which shows the pyrophosphatase activity in normal condition, decreased according to atrophy of its epithelium, and reaction of Henle's loop, papillary dut and collecting tubule were increased. (2) I could not find the histological and histochemic a l changes on the opposite kidney. (3) Sometimes reaction of interstitial fibrous tissue that was often negative in n ormal condition showed the weak positive. (4) I could not find the changes of wall of blood vessels. Chapter II. Change of pyrophosphatase activity in r abbit's kidney under injection of corrosive sublimate. In this chapter I investigated the change of activity in rabbit's kidney under injection of corrosive sublimate. (1) Pyrop h osphatase activity: of cells and tissue that suggest the morphological changes decreased clearly. (2) Decrease o f pyrophosphatase activity often appeared before morphological changes, that is, I recognized functional disorder before morphological changes. (3) Functional compensation exist between collecting tub u le and distal or proximal convuluted tubule. (4) Ren a l disturbance results from injection of corrosive sublimate are more stronger in distal or proximal convuluted tubules than glomerulus. (5) Activity in new cells was stronger tha n injured cells. Chapter III. Change of pyrophosphatase activity in rabbit's kidney under injection of potassium dichromate. In this chapter I discussed the change of activity in cause of injection of potassium dichr omste. Co n sequently I could not find more difference between II and III chapter.