尿中下垂体性性腺刺戟ホルモンの定量に関する研究

Abstract

Since the quantitative analysis of the pituitary gonadotropin in h u man urine revealed to be applicable for clinical diagnosis, various methods to measure urinary gonadotropin have been presented. However these methods have some faults in troublesome experimental manipulations, considerable loss in the process of extraction or toxity of the final products. The present study was performed to fmd a simple and reliable method for clinical use, and the following results were obtained. 1. Kaolin adsorption, becaus e of its reliability and practibility, is a method of choice for extraction of gonadotropin from urine. 2. But, even in this method, ther e are some faults that procedure is rather complicated and the yield of gonadotropin is influenced to some extent by the quality of kaolin. 3. The process of extraction has become simple by use of crude kaolin. T h ere is no substantial difference in extraction, be the kaolin crude or purified. 4. Synthetic weak alkaline ion exchanger resin which i s of more uniform quality comparing to kaolin, are recommended for extraction. 5. Various methods for measuring a c tivity of thus extracted gonadotropin were studied, and it was concluded that the best assay method for FSH, ICSH and total gonadotropin are respectively as follows to weigh the increase of the ovarian weight of the intact immature mouse treated with HCG, to measure the enlargement of the prostate and seminal vesicles of the hypophysectomized immature rats, and to calculate the increase of the uterine weight of the intact mouse. 6. Prolacti n in the urine was extracted by the alcohol precipitating method and assayed with pigeon's crop gland method. It was observed that in t h e antiandrogenic therapy for the prostatic cancer, there might exist corelation between prolactin output and effectiveness of the treatment , More basic researches would be necessary in order to complete the method of quantitative analysis of prolactin.