in vitro BrdU標識法と抗BrdUモノクローナル抗体を用いた尿路性器腫瘍の細胞動態学的研究

Abstract

in vitro BrdU標識法と抗BrdU抗体による細胞動態解析法を用いて, 泌尿器系腫瘍20例の細胞動態を解析した.1)全症例において, BrdUを取り込んだDNA合成期の細胞は明瞭に染色されており, 標識率(LI)の算定は容易であった.2)尿路移行上皮癌14例において, LIと年齢, 組織学的分化度, 深達度との関係をみたところ, 年齢とは一定の関係を認めなかったが, G1のlow grade群とG2およびG3のhigh grade群間には有意差を認めた(P<0.05).また, pT1とpT3群間のLIにも有意差を認め(P<0.05), 全体としては深達度が増すにつれLIも大きくなる傾向にあった

With the aim to apply a rapid analytic method of cell kinetics using in vitro BrdU labelling and anti-BrdU monoclonal antibody to the clinical fields, we investigated the cell kinetics of 20 urogenital malignant tumors. The DNA synthesizing cells which take up BrdU in its nuclei can be detected so clearly, since the labelling index (LI) is easily identified. The correlations between LI and age and with histological grade and stage of 14 transitional cell cancers of urinary tract were investigated. There was no correlation between LI and age. The LI of high grade groups (G2 & G3) were significantly higher than that of the low grade group (G1) (p less than 0.05). Also between LI of pT1 group and pT3 group, there was a statistical significance (p less than 0.05) and generally LI tended to increase according to stage progression. Therefore, the cytokinetic data obtained from the before mentioned method can be utilized as a useful marker for measuring malignant potentials of each tumor.