Flow Cytometryを用いた精巣腫瘍パラフィンブロックによるPloidyの検討

Abstract

精巣腫瘍36例のパラフィンブロックを用いて, FCMによる核DNA量解析を行った.1) DNAヒストグラムは36例中20例で解析可能であったが, teratomaでは他の組織型に比べて解析不能症例が多かった.2) DNA aneuploidyの出現は20例中11例にみられたが, seminomaとnon-seminomaで組織型の違いによる差はなかった.3) seminomaはstageの進行に伴いaneuploidの出現頻度が高くなった.4) HCG-β値の上昇を認めたseminomaでは, 4例中3例にaneuploidがみられmalignant potentialが高いと推定された

The DNA content of paraffin-embedded materials was determined retrospectively using flow cytometry (FCM) in 36 germ cell tumors, and related to histological type, clinical staging and tumor marker. These histograms were classified from the basis of mode and variance into a diploid and an aneuploid pattern. We could evaluate the DNA histograms in 20 of 36 specimens (56%). Aneuploid patterns were found in 11 of 20 evaluated cases, but there was no correlation between ploidy patterns and histological types. Aneuploid patterns were demonstrated in 2 of 6 stage I cases (33%), and 4 of 5 stage II cases (80%) in seminomas. The difference between stage I and II cases was not statistically significant. There was no correlation between clinical staging and DNA content in non-seminomas. Of the seminomas with elevated human chorionic gonadotropin (HCG) titers, 3 of 4 cases showed aneuploid patterns. These findings indicate that the determination of DNA ploidy in seminomas may prove to be of prognostic value.