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Title: Proliferating Cell Nuclear Antigen(PCNA)の表在性膀胱癌組織における発現 : パラフィン切片での応用
Other Titles: Expression of proliferating cell nuclear antigen in superficial bladder cancer--application to paraffin-embedded tissue sections
Authors: 和田, 尚  KAKEN_name
島袋, 智之  KAKEN_name
栗栖, 弘明  KAKEN_name
Shameem, Isteaq A.
吉弘, 悟  KAKEN_name
松山, 豪泰  KAKEN_name
内藤, 克輔  KAKEN_name
橋本, 治  KAKEN_name
田中, 一成  KAKEN_name
Author's alias: Wada, Takashi
Shimabukuro, Tomoyuki
Kurisu, Hiroaki
Shameem, Isteaq A.
Yoshihiro, Satoru
Matsuyama, Hideyasu
Naito, Katsusuke
Hashimoto, Osamu
Tanaka, Kazunari
Keywords: PCNA
Bladder cancer
Paraffin-embedded tissue
Issue Date: Apr-1993
Publisher: 泌尿器科紀要刊行会
Journal title: 泌尿器科紀要
Volume: 39
Issue: 4
Start page: 313
End page: 319
Abstract: 抗PCNAモノクローナル抗体を用いて膀胱癌パラフィン包埋組織の免疫組織化学染色を行った。1)全56症例においてPCNA陽性細胞を検出でき,PCNA陽性率は平均34.0%であった。2)PCNA陽性率と腫瘍数との関係では,単発群(n=22)の平均24.8%に比較して,多発群(n=34)は40.0%と有意に高値であった。3)PCNA陽性率と組織学的異型度との関係では,grade 1群(n=19)の平均15.6%に比較してgrade 2群(n=27)は39.9%,grade 3群(n=10)は53.1%と有意に高値であった。一方,組織学的深達度とPCNA陽性率との間には,有意な関連性は認められなかった。4)PCNA陽性率34%異常の群では,PGNA陽性率34%未満の群に比較して有意に再発率か高かった。5)PCNA陽性率は,腫瘍数,組織学的異型度と高い相関を認めることより,膀胱癌の生物学的悪性度を知る新しい指標となりえる可能性が示唆された
It is important to know the proliferating ability and the malignant potential of each tumor. We studied 56 cases of pTa to pT1 superficial bladder tumors using immunohistochemical staining for proliferating cell nuclear antigen (PCNA), and compared the results with the clinical course of each patient. We obtained the following results. 1) We detected the PCNA positive nuclei in all cases, and the PCNA positive rates varied within a range of 1.1-77.5% with a mean of 34.0%. 2) The PCNA positive rate showed no correlation with age, sex, duration of paraffin-embedded, or pathological stage, but showed a significant correlation with the number of tumors, pathological grade of malignancy, or non-recurrence rate. PCNA positive rate of Grade 1 cases (n = 19, 15.6%: mean) was significantly lower than those of Grade 2 cases (n = 27, 39.9%) or Grade 3 cases (n = 10, 53.1%) (P < 0.01). The recurrence rate of the cases with PCNA positive rates of more than 34% (n = 24) was significantly higher than that of the cases with a PCNA positive rate of less than 34% (n = 32) (P < 0.05). In conclusion, the method of counting the rate of PCNA positive nuclei is considered to be very useful because of its applicability to paraffin-embedded tissue sections and the simple and rapid techniques. Our results in bladder cancer tissues suggest that this method may also be useful for investigating the proliferating ability and the malignant potential of tumors in general.
PubMed ID: 8099249
Appears in Collections:Vol.39 No.4

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