膀胱腫瘍の組織化学的研究

Abstract

膀胱腫瘍27例, 尿管腫瘍3例, 腎盂腫瘍1例の合計31例の尿路上皮腫瘍を対象とし, 組織学的に酸性ムコ多糖の検出, 同定を行うためアルシアン青染色を中心として行い, 各種の酵素消化法を用いて検索した.(1)それぞれ若干の差はあるが全ての腫瘍組織の間質に酸性ムコ多糖が認められた.(2)腫瘍のgradeの低い方が, ヒアルロン酸の割合が比較的多かった.なお, 扁平上皮癌ではヒアルロン酸の量が相対的に少なかった.(3)high stage例ではコンドロイチン硫酸A, Cが多量に認められ, ヒアルロン酸は相対的に少なかった.デルマタン酸はhigh stageになるほど多くなる傾向が認められた

Twenty seven bladder tumors, three ureteral tumors and one renal pelvic tumor were studied by means of light microscopic histochemical methods for demonstration and identification of acid mucopolysaccharides. Alcian blue (pH 1.0), alcian blue (pH 2.5), periodic acid-Schiff (PAS) and aldehyde-fuchusin stainings were performed. These stainings showed that all tumor specimens contained acid mucopolysaccharides. For identifying individual acid mucopolysaccharides, enzyme digestion procedures were performed prior to staining with alcian blue. (streptomyces hyaluronidase, testicular hyaluronidase, chondroitinase ABC, chondroitinase AC, keratanase, heparinase, heparitinase.) According to these experiments, high-grade, and high-stage tumors contained large amounts of sulfated mucopolysaccharides. Squamous cell carcinomas of the bladder contained especially large amounts of chondroitin sulfate AC.