新しいシスチン尿症診断キットについて

Abstract

A new screening method for cystinuria is presented in this paper. The method is so simple that the diagnosis can be made only by pouring 4 ml of urine into the reagent. Reactions are as follows: Cystine is reduced to cysteine with Na2S2O4 in the presence of Ni2+, and then cysteine forms the mixed ligand complex with Ni2+ and S2-. The reagent is so adjusted to be sensitive at 50µg/ml or higher cystine concentration. Although not specific for cystine but for other thiols, the clinical results accord with those of the cyanide nitroprusside method both in false positive and false negative. The method is characterized by such points that no pretreatment of urine is necessary and that results is obtained so quickly within three minutes. Also it is more safe without such a dangerous reagent as cyanide.