STUDIES ON THE FIBRINOLYTIC ACTIVITY IN CANCEROUS BLADDER TISSUES : A STUDY BY GEL FILTRATION

Abstract

膀胱癌組織抽出液はフイブリン平板法において線溶活性を示さず, urokinase inhibitorの約250units/ml を含有したゲル濾過法(Sephadex G-200) を用いて, この抽出液中にfibrinolysisactivators とinhibitorsの存在することが判明した。ヒト正常膀胱からの粗製tissueactivatorはこの癌抽出液により活性が抑制された。 urokinaseは同じ抽出液により高度に抑制され，urokinase 30 Ploug unitsはこの抽出液中に認められたurokinase inhibitor 250 unitsによりほとんど完全に抑制された。一方この同じ抽出液の量により，tissue activator 8 Ploug unitsはその30%のみが抑制されたにすぎなかった。正常膀胱組織抽出液はplasminogen activatorによる中等度の線、溶活性を示すとともに，urokinaseに対してはきわめて軽度の抑制効果(約6units/ml)を示した。

The extract from cancerous bladder tissue was fibrinolytically inactive in fibrin plate assay and contained approximately 250 units per ml of urokinase inhibitor. By means of gel filtration (Sephadex G-200) the activators and inhibitors of fibrinolysis contained in the extract were separated. Tissue activator partially purified from a normal human bladder was inhibited by cancerous bladder tissue extract. To a much greater extent urokinase was inhibited by the same extract, specifically, 30 Ploug units of urokinase were almost completely inhibited by the 250 units of urokinase inhibitor present in the extract, whereas only 30 per cent of the 8 Ploug units of tissue activator was inhibited by the same amount of extract. The normal bladder tissue extract showed moderate fibrinolytic activity caused by an activator of plasminogen and very slight inhibitory activity (approximately 6 units per ml) against urokinase.