膀胱ロイコプラキーの電子顕微鏡的観察 とくにvirus-like particlesについて

Abstract

Transurethral biopsy was performed on twelve cases of leukoplakia of the urinary bladder (type I of Anderson's classification). The tissues were primarily fixed in cacodylate-buffered 3.0% glutaraldehyde and then finally fixed in osmic acid each for one hour, dehydrated in graded concentrations of ethanol, and embedded in Epon 812. Thin sections were stained with uranyl acetate and lead acetate. Observations and photography were performed on type HS-7 and HD -12 electron microscopes. Results and discussions could be summarized as follows ; 1. Nucleus of bladder epithelial cells was enlarged and the outline of the membrane was irregularly changed and pseudonuclear inclusions were observed in 10 cases. 2. The shape of nucleolus was irregular and its enlargement or plurality were found in 11 cases. 3. Nuclear body was found in all cases, which was composed of amorphous protein without limiting membrane. 4. Mitochondria increased in number, and their deformity or gathering were found. These findings might indicate precancerous change electron microscopically. 5. Virus-like particles were observed in some epithelial cell cytoplasm in 4 cases, which were similar to cored vesicles and about 80nm in diameter. These particles resembled to the mouse mammary viruses ultrastructurally. A virus-like particle had fine 20 subunit structures when observed with the rotation technique, strongly suggesting virus particles, although further observations are needed.