尿路性器癌患者の細胞性免疫能に関する研究 第1報: PHAによる末梢リンパ球幼若化現象の基礎的検討

Abstract

For the purpose of evaluating the cell-mediated immunocompetence in patients with urogenital cancer, observations of PHA induced blastoid transformations of peripheral lymphocytes were attempted. In this report, dose-and time-response of this phenomenon in lymphocytes of the healthy adults and the effects of addition of some kinds of sera on those responses were investigated. 1. The effects of PHA-P concentrations. In the lymphocyte culture with medium containing 20% AB-serum, 0.1 ml of PHA-P diluted to 10×, 100×, 400× and 800 with the medium was added and the blastoid transformations were periodically observed. At the 2rd day of culture, the response was developed at the maximum level in each group. The responses were significantly higher in 100 X and 400× concentration than in 10× or 800×concentration of PHA-P. 2. The effects of some kinds of sera added. In the lymphocyte culture with medium containing 400× diluted PHA-P, the responses, at the 3rd day, were highest in addition of FCS and AB-serum, auto-serum were followed. The responses were slightly higher in medium with inactivated sera than in those with non-inactivated sera. However, there was no significant difference. Therefore, it is most adequate that the blastoid transformation is observed in the medium containing with 100× or 400× diluted PHA-P and inactivated AB-serum at the 3rd day of culture.