Access count of this item: 154
|Other Titles:||CHARACTERISTICS OF ATPase IN THE MICROSOMAL MEMBRANE OF THE RABBIT KIDNEY AND BLADDER : CHANGE AFTER SPINAL INJURY AND EFFECT OF ROBAVERON AN ANALYSIS OF ATPase ACTIVITY DISTRIBLTTION BY MEANS OF SUCROSE DENSITY GRADIENT METHOD|
|Authors:||下平, 正文 |
|Author's alias:||Shimohira, Masafumi|
|Abstract:||ATPase in the microsomal membrane of the rabbit kidney and bladder and the related biological macromolecules such as protein and nucleic acid were submitted for the analysis with the sucrose density gradient method in order to know their in vitro localization and distribution. On the other hand, the in vivo experiment was carried out, with the above group being a standard model, on the rabbits with experimentally induced spinal injury and those with such injury treated with Robaveron. The following results were obtained. 1 ) ATPase localized in the membrane of organelles such as microsomes, mitochondria, granules, lysosomes and their upper fraction showed the distribution of the regular fashion corresponding well to the protein fractions. 2) ATPase of the microsomal membrane of the kidney and bladder, namely 48,000×g fraction, showed a localization of a single peak exactly corresponding to the distribution of other membrane biological macromolecules such as protein and nucleic acids. This distribution changed to the dual peak pattern under the action of Brij 58 showing the elevation of the relative activity of ATPase. Two peaks consisted of the low density fraction and the upper layer fraction. On the other hand, this enzyme was shifted, under the presence of DOC, to the fraction of the heavy specific gravity accompanied by formation of a new fraction of low density. 3) In animals with injury at the low spinal cord the kidney ATPase and the microsomal biological macromolecules moved to the low density area just as seen in the addition of Brij 58 to the normal rabbit. Administration of Robaveron resulted in restoration to the normal pattern. 4) Hemoglobin-like pigment appearing in the supernatant fraction of the kidney and bladder microsome after spinal cord injury, either the high or low, was effectively inhibited by means of administration of Robaveron. 5) Microsomal (Na+ - K+) ATPase was partly purified in the animals, normal, spinal cord injury and Robaveron-treated. The relative activity of this enzyme of these three groups showed no significant difference. From the results as above, therapeutic effect of Robaveron for the difficulty in urination in spinal cord injury was proved to be related with functional repair of the membrane microsome of the kidney and bladder.|
|Appears in Collections:||Vol.24 No.10|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.