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Title: 抗菌剤の膀胱腔内への移行にかんする研究 2.TLC-bioautographyによる抗菌活性代謝産物の検索について
Other Titles: STUDIES ON THE TRANSFER OF ANTIBACTERIAL AGENTS INTO THE INTRAVESICAL SPACE 2. INVESTIGATION OF THE ACTIVE METABOLITES BY MEANS OF THIN-LAYER CHROMATOGRAPHIC BIOAUTOGRAPHY
Authors: 上領, 頼啓  KAKEN_name
Author's alias: Kamiryo, Yoriaki
Issue Date: Oct-1978
Publisher: 泌尿器科紀要刊行会
Journal title: 泌尿器科紀要
Volume: 24
Issue: 10
Start page: 819
End page: 825
Abstract: Male rats, weighing 300 to 350 grams, were nephrectomized bilaterally and their penis was ligated. The bladder was instilled with 0.7 ml of normal saline. This was designated as the intravesical fluid. Antibacterial agents such as kanamycin, DKB, gentamicin, cefamezin, rifampicin and nalidixic acid were administered to these rats 100 mg per 1 kg body weight. Forty-eight hours later, serum and intravesical fluid were submitted for thin-layer chromatographic bioautography. This procedure was made also at 24 hours after administration in the nalidix acid group. No active metabolite was detected in kanamycin, gentamicin, cefamezin and rifampicin. In DKB, serum sample occasionally showed the small inhibitory zone of a different Rf value from the original DKB, but an exact proof of being an active metabolite could not be obtained. In nalidixic acid, 24 hours samples, both serum and intravesical fluid, showed nalidixic acid as well as hydroxynalidixic acid. In 48 hours samples, both serum and the intravesical fluid, nalidixic acid could not be detected and only hydroxy-nalidixic acid was identified.
URI: http://hdl.handle.net/2433/122275
Appears in Collections:Vol.24 No.10

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