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タイトル: | 尿中トリプシンインヒビター(UTI)に関する研究 -UTIの新定量法について- |
その他のタイトル: | STUDIES ON URINARY TRYPSIN INHIBITOR (UTI) - A NEW DETERMINATION METHOD OF UTI - |
著者: | 土岐, 尚親 ![]() 林, 睦雄 ![]() 前原, 進 ![]() 須見, 洋行 ![]() |
著者名の別形: | Toki, Naochika Hayashi, Mutsuo Maehara, Susumu Sumi, Hiroyuki |
発行日: | Dec-1978 |
出版者: | 京都大学医学部泌尿器科学教室 |
誌名: | 泌尿器科紀要 |
巻: | 24 |
号: | 12 |
開始ページ: | 1053 |
終了ページ: | 1060 |
抄録: | A new determination of urinary trypsin inhibitor (UTI) was developed from casein-plate method. We studied the relationship between the activity level of UTI by the present method and the activity level of urokinase (UK) by fibrin agar-plate method in various renal diseases. A sample of trypsin solution (Sigma, type II, 25 mM Ca2+) was adsorbed to a filter paper (5.8×5.8 mm), and lyophilized to be fixed. Then 5 pi of urine was adsorbed to the above. It was applied on 0.05% casein-plate (10 mM Ca2+), and incubated at 37"C. UTI activity level was determined in lysis area of trypsin. The results were well correlated with those by the test-tube method already reported. The procedure of the present method made it possible to test more simply compared with conventional methods. The amount to inhibit 1 pg of trypsin was assigned to be one unit, as for UTI activity. As for UK level in urine, UK was determined by fibrin agar-plate method with commercial UK (Green Cross Co. Ltd.) as the standard. The results are as follows: 1. Normal human urine contains about 4.1 units of UTI and 11.1 IU of UK per ml. UTI/UK is approximately 0.4. |
URI: | http://hdl.handle.net/2433/122300 |
出現コレクション: | Vol.24 No.12 |
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