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タイトル: | Methylglyoxal activates Gcn2 to phosphorylate eIF2α independently of the TOR pathway in Saccharomyces cerevisiae. |
著者: | Nomura, Wataru Maeta, Kazuhiro Kita, Keiko Izawa, Shingo Inoue, Yoshiharu |
著者名の別形: | 井上, 善晴 |
キーワード: | Methylglyoxal TORC1 Gcn2 eIF2α S. cerevisiae Rapamycin |
発行日: | May-2010 |
出版者: | Springer |
誌名: | Applied microbiology and biotechnology |
巻: | 86 |
号: | 6 |
開始ページ: | 1887 |
終了ページ: | 1894 |
抄録: | Methylglyoxal is a ubiquitous 2-oxoaldehyde derived from glycolysis. Previously, we have reported that methylglyoxal attenuates the rate of overall protein synthesis in Saccharomyces cerevisiae through phosphorylation of the alpha subunit of translation initiation factor 2 (eIF2alpha) in a Gcn2-dependent manner. Phosphorylation of eIF2alpha impedes the formation of a translation initiation complex, and subsequently, overall protein synthesis is reduced. Uncharged tRNA plays an important role in the activation of Gcn2, although we found that MG treatment did not elevate the levels of uncharged tRNA. Rapamycin, a potent inhibitor of TOR kinase, is known to induce phosphorylation of eIF2alpha without affecting the levels of uncharged tRNA. We determined the correlation between methylglyoxal and TOR kinase activity and found that phosphorylation of eIF2alpha by methylglyoxal occurred independently of the target of rapamycin (TOR) pathway. |
著作権等: | The original publication is available at www.springerlink.com This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/123517 |
DOI(出版社版): | 10.1007/s00253-009-2411-z |
PubMed ID: | 20077113 |
出現コレクション: | 学術雑誌掲載論文等 |
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