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| タイトル: | Crystallization and preliminary X-ray analysis of L-azetidine-2-carboxylate hydrolase from Pseudomonas sp. strain A2C. |
| 著者: | Toyoda, Mayuko Jitsumori, Keiji Mikami, Bunzo   Wackett, Lawrence P Kurihara, Tatsuo  https://orcid.org/0000-0002-7777-1378 (unconfirmed)Esaki, Nobuyoshi |
| 著者名の別形: | 栗原, 達夫 |
| キーワード: | L-Azetidine-2-carboxylate hydrolase Pseudomonas sp. strain A2C |
| 発行日: | 1-Jul-2010 |
| 出版者: | International Union of Crystallography |
| 誌名: | Acta crystallographica. Section F, Structural biology and crystallization communications |
| 巻: | 66 |
| 号: | Pt 7 |
| 開始ページ: | 801 |
| 終了ページ: | 804 |
| 抄録: | L-Azetidine-2-carboxylate hydrolase from Pseudomonas sp. strain A2C catalyzes a ring-opening reaction that detoxifies L-azetidine-2-carboxylate, an analogue of L-proline. Recombinant L-azetidine-2-carboxylate hydrolase was overexpressed, purified and crystallized using polyethylene glycol and magnesium acetate as precipitants. The needle-shaped crystal belonged to space group P2(1), with unit-cell parameters a = 35.6, b = 63.6, c = 54.7 A, beta = 105.5 degrees . The crystal diffracted to a resolution of 1.38 A. The calculated V(M) value was 2.2 A(3) Da(-1), suggesting that the crystal contains one enzyme subunit in the asymmetric unit. |
| 著作権等: | © 2010 International Union of Crystallography |
| URI: | http://hdl.handle.net/2433/126663 |
| DOI(出版社版): | 10.1107/S1744309110017045 |
| PubMed ID: | 20606277 |
| 出現コレクション: | 学術雑誌掲載論文等 |
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