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j.biomaterials.2010.06.058.pdf1.13 MBAdobe PDF見る/開く
タイトル: Development of an ultrasound-responsive and mannose-modified gene carrier for DNA vaccine therapy.
著者: Un, Keita
Kawakami, Shigeru
Suzuki, Ryo
Maruyama, Kazuo
Yamashita, Fumiyoshi  kyouindb  KAKEN_id
Hashida, Mitsuru  kyouindb  KAKEN_id
著者名の別形: 運, 敬太
川上, 茂
橋田, 充
キーワード: Gene transfer
Bubble lipoplexes
Ultrasound exposure
Mannose receptors
Antigen presenting cells
DNA vaccine therapy
発行日: Oct-2010
出版者: Elsevier Ltd
誌名: Biomaterials
巻: 31
号: 30
開始ページ: 7813
終了ページ: 7826
抄録: Development of a gene delivery system to transfer the gene of interest selectively and efficiently into targeted cells is essential for achievement of sufficient therapeutic effects by gene therapy. Here, we succeeded in developing the gene transfection method using ultrasound (US)-responsive and mannose-modified gene carriers, named Man-PEG(2000) bubble lipoplexes. Compared with the conventional lipofection method using mannose-modified carriers, this transfection method using Man-PEG(2000) bubble lipoplexes and US exposure enabled approximately 500-800-fold higher gene expressions in the antigen presenting cells (APCs) selectively in vivo. This enhanced gene expression was contributed by the improvement of delivering efficiency of nucleic acids to the targeted organs, and by the increase of introducing efficiency of nucleic acids into the cytoplasm followed by US exposure. Moreover, high anti-tumor effects were demonstrated by applying this method to DNA vaccine therapy using ovalbumin (OVA)-expressing plasmid DNA (pDNA). This US-responsive and cell-specific gene delivery system can be widely applied to medical treatments such as vaccine therapy and anti-inflammation therapy, which its targeted cells are APCs, and our findings may help in establishing innovative methods for in-vivo gene delivery to overcome the poor introducing efficiency of carriers into cytoplasm which the major obstacle associated with gene delivery by non-viral carriers.
著作権等: © 2010 Elsevier Ltd
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/126738
DOI(出版社版): 10.1016/j.biomaterials.2010.06.058
PubMed ID: 20656348
出現コレクション:学術雑誌掲載論文等

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