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タイトル: | The optimal activity of a pseudozymogen form of recombinant matriptase under the mildly acidic pH and low ionic strength conditions. |
著者: | Inouye, Kuniyo Yasumoto, Makoto Tsuzuki, Satoshi https://orcid.org/0000-0003-4385-0075 (unconfirmed) Mochida, Seiya Fushiki, Tohru |
著者名の別形: | 井上, 國世 |
発行日: | Apr-2010 |
出版者: | Oxford University Press on behalf of the Japanese Biochemical Society |
誌名: | Journal of biochemistry |
巻: | 147 |
号: | 4 |
開始ページ: | 485 |
終了ページ: | 492 |
抄録: | Matriptase is a transmembrane serine protease that is strongly expressed in epithelial cells. The single-chain zymogen of matriptase is considered to have inherent activity, leading to its own activation (i.e. conversion to the disulphide-linked-two-chain form by cleavage after Thr-Lys-Gln-Ala-Arg614). Also, there is growing evidence that the activation of zymogen occurs at the cell surface and in relation to the acidification and lowering of ionic strength within cell-surface microenvironments. The present study aimed to provide evidence for the involvement of zymogen activity in its activation in physiologically relevant cellular contexts. For this purpose, the activity of a pseudozymogen form of recombinant matriptase (HL-matriptase zymogen) was examined using acetyl-l-Lys-l-Thr-l-Lys-l-Gln-l-Leu-l-Arg-4-methyl-coumaryl-7-amide as a substrate. HL-matriptase zymogen exhibited optimal activity toward the substrate pH approximately 6.0. The substrate hydrolysis at the pH value was hardly detected when NaCl was present at a concentration of 145 mM. In a buffer of pH 6.0 containing 5 mM NaCl, the activity of HL-matriptase zymogen was only approximately 30-times lower than that of the respective two-chain form. These findings suggest that the in vivo activation of matriptase zymogen occurs via a mechanism involving the zymogen activity. |
著作権等: | © The Authors 2009. Published by Oxford University Press on behalf of the Japanese Biochemical Society. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/128777 |
DOI(出版社版): | 10.1093/jb/mvp190 |
PubMed ID: | 19919953 |
出現コレクション: | 学術雑誌掲載論文等 |
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