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Title: Influence of manipulating hypoxia in solid tumors on the radiation dose-rate effect in vivo, with reference to that in the quiescent cell population.
Authors: Masunaga, Shin-ichiro  kyouindb  KAKEN_id
Hirayama, Ryoichi
Uzawa, Akiko
Kashino, Genro
Takata, Takushi  kyouindb  KAKEN_id
Tanaka, Hiroki  kyouindb  KAKEN_id
Suzuki, Minoru  kyouindb  KAKEN_id  orcid (unconfirmed)
Kinashi, Yuko  kyouindb  KAKEN_id
Liu, Yong
Koike, Sachiko
Ando, Koichi
Ono, Koji
Author's alias: 増永, 慎一郎
Keywords: Dose-rate effect
Manipulating hypoxia
Quiescent cell
Carbon-ion beams
Issue Date: Feb-2010
Publisher: Springer Verlag
Journal title: Japanese journal of radiology
Volume: 28
Issue: 2
Start page: 132
End page: 142
Abstract: PURPOSE: The aim of this study was to clarify the effect of manipulating intratumor hypoxia on radiosensitivity under reduced dose-rate (RDR) irradiation. MATERIALS AND METHODS: Tumor-bearing mice were continuously given 5-bromo-2'-deoxyuridine (BrdU) to label all proliferating (P) cells. They received gamma-rays or accelerated carbon-ion beams at high dose-rate (HDR) or RDR with or without tumor clamping to induce hypoxia. Some mice without clamping received nicotinamide, an acute hypoxia-releasing agent or misonidazole, a hypoxic cell radio-sensitizer before irradiation. The responses of quiescent (Q) and total (= P + Q) cells were assessed by the micronucleus frequency using immunofluorescence staining for BrdU. RESULTS: The clearer decrease in radiosensitivity in Q than total cells after RDR gamma-ray irradiation was suppressed with carbon-ion beams, especially with a higher linear energy transfer value. Repressing the decrease in the radiosensitivity under RDR irradiation through keeping tumors hypoxic during irradiation and enhancing the decrease in the radiosensitivity by nicotinamide were clearer with gamma-rays and in total cells than with carbon-ion beams and in Q cells, respectively. Inhibiting the decrease in the radiosensitivity by misonidazole was clearer with gamma-rays and in Q cells than with carbon-ion beams and in total cells, respectively. CONCLUSION: Manipulating hypoxia during RDR as well as HDR irradiation influences tumor radiosensitivity, especially with gamma-rays.
Rights: The original publication is available at
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
DOI(Published Version): 10.1007/s11604-009-0397-1
PubMed ID: 20182848
Appears in Collections:Journal Articles

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