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タイトル: | Synthesis of a new NIR fluorescent Nd complex labeling agent. |
著者: | Aita, Kazuki Temma, Takashi Shimizu, Yoichi Kuge, Yuji Seki, Koh-Ichi Saji, Hideo |
著者名の別形: | 天満, 敬 佐治, 英郎 |
キーワード: | Neodymium Near-infrared Fluorescent labeling Maleimide |
発行日: | Jan-2010 |
出版者: | Springer |
誌名: | Journal of fluorescence |
巻: | 20 |
号: | 1 |
開始ページ: | 225 |
終了ページ: | 234 |
抄録: | Fluorescent analysis has been widely used in biological, chemical and analytical research. A useful fluorescent labeling agent should include NIR emission, a large Stoke's shift, and good labeling ability without interfering with the pharmacological profile of the labeled compound. Thus, we planned to develop an M-AMF-DOTA(Nd) derivative composed of an NIR fluorescent moiety and a maleimide conjugating moiety as a new NIR fluorescent labeling agent which fulfills these requirements. M-AMF-DOTA(Nd) was synthesized from 4-amino-fluorescein and was conjugated with an avidin molecule (Avidin-AMF-DOTA(Nd)) through Lys-side chains by reaction with 2-iminothiolane. The fluorescent features of M-AMF-DOTA(Nd) and Avidin-AMF-DOTA(Nd) were comparatively evaluated. A binding assay of Avidin-AMF-DOTA(Nd) with D-biotin and a tumor cell-uptake study were performed to estimate the effects of conjugation on the biological and physicochemical features of the protein. M-AMF-DOTA(Nd) was obtained in 22% overall yield. M-AMF-DOTA(Nd) had a typical NIR fluorescence from the Nd ion (880 nm and 900 nm from 488 nm excitation). Avidin-AMF-DOTA(Nd) was easily synthesized and also had typical NIR fluorescence from the Nd ion without loss of fluorescent intensity. The binding affinity of Avidin-AMF-DOTA(Nd) to D-biotin was equivalent to naive avidin. Avidin-AMF-DOTA(Nd) was taken up by tumor cells in the same manner as avidin conjugated with fluorescein isothiocyanate, an established, widely used fluorescent avidin. Results from this study indicate that M-AMF-DOTA(Nd) is a potential labeling agent for routine NIR fluorescent analysis. |
著作権等: | The original publication is available at www.springerlink.com この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/128929 |
DOI(出版社版): | 10.1007/s10895-009-0542-3 |
PubMed ID: | 19821013 |
出現コレクション: | 学術雑誌掲載論文等 |
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