ダウンロード数: 508
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
s10895-009-0542-3.pdf | 307.74 kB | Adobe PDF | 見る/開く |
完全メタデータレコード
DCフィールド | 値 | 言語 |
---|---|---|
dc.contributor.author | Aita, Kazuki | en |
dc.contributor.author | Temma, Takashi | en |
dc.contributor.author | Shimizu, Yoichi | en |
dc.contributor.author | Kuge, Yuji | en |
dc.contributor.author | Seki, Koh-Ichi | en |
dc.contributor.author | Saji, Hideo | en |
dc.contributor.alternative | 天満, 敬 | ja |
dc.contributor.alternative | 佐治, 英郎 | ja |
dc.date.accessioned | 2010-10-21T05:08:29Z | - |
dc.date.available | 2010-10-21T05:08:29Z | - |
dc.date.issued | 2010-01 | - |
dc.identifier.issn | 1053-0509 | - |
dc.identifier.uri | http://hdl.handle.net/2433/128929 | - |
dc.description.abstract | Fluorescent analysis has been widely used in biological, chemical and analytical research. A useful fluorescent labeling agent should include NIR emission, a large Stoke's shift, and good labeling ability without interfering with the pharmacological profile of the labeled compound. Thus, we planned to develop an M-AMF-DOTA(Nd) derivative composed of an NIR fluorescent moiety and a maleimide conjugating moiety as a new NIR fluorescent labeling agent which fulfills these requirements. M-AMF-DOTA(Nd) was synthesized from 4-amino-fluorescein and was conjugated with an avidin molecule (Avidin-AMF-DOTA(Nd)) through Lys-side chains by reaction with 2-iminothiolane. The fluorescent features of M-AMF-DOTA(Nd) and Avidin-AMF-DOTA(Nd) were comparatively evaluated. A binding assay of Avidin-AMF-DOTA(Nd) with D-biotin and a tumor cell-uptake study were performed to estimate the effects of conjugation on the biological and physicochemical features of the protein. M-AMF-DOTA(Nd) was obtained in 22% overall yield. M-AMF-DOTA(Nd) had a typical NIR fluorescence from the Nd ion (880 nm and 900 nm from 488 nm excitation). Avidin-AMF-DOTA(Nd) was easily synthesized and also had typical NIR fluorescence from the Nd ion without loss of fluorescent intensity. The binding affinity of Avidin-AMF-DOTA(Nd) to D-biotin was equivalent to naive avidin. Avidin-AMF-DOTA(Nd) was taken up by tumor cells in the same manner as avidin conjugated with fluorescein isothiocyanate, an established, widely used fluorescent avidin. Results from this study indicate that M-AMF-DOTA(Nd) is a potential labeling agent for routine NIR fluorescent analysis. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Springer | en |
dc.rights | The original publication is available at www.springerlink.com | en |
dc.rights | この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 | ja |
dc.rights | This is not the published version. Please cite only the published version. | en |
dc.subject | Neodymium | en |
dc.subject | Near-infrared | en |
dc.subject | Fluorescent labeling | en |
dc.subject | Maleimide | en |
dc.subject.mesh | Animals | en |
dc.subject.mesh | Avidin/metabolism | en |
dc.subject.mesh | Biological Transport | en |
dc.subject.mesh | Biotin/metabolism | en |
dc.subject.mesh | Cell Line, Tumor | en |
dc.subject.mesh | Fluorescent Dyes/chemical synthesis | en |
dc.subject.mesh | Fluorescent Dyes/chemistry | en |
dc.subject.mesh | Fluorescent Dyes/metabolism | en |
dc.subject.mesh | Heterocyclic Compounds, 1-Ring/chemistry | en |
dc.subject.mesh | Infrared Rays | en |
dc.subject.mesh | Maleimides/chemistry | en |
dc.subject.mesh | Neodymium/chemistry | en |
dc.subject.mesh | Organometallic Compounds/chemical synthesis | en |
dc.subject.mesh | Organometallic Compounds/chemistry | en |
dc.subject.mesh | Organometallic Compounds/metabolism | en |
dc.subject.mesh | Rats | en |
dc.subject.mesh | Spectrometry, Fluorescence | en |
dc.title | Synthesis of a new NIR fluorescent Nd complex labeling agent. | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.ncid | AA10871550 | - |
dc.identifier.jtitle | Journal of fluorescence | en |
dc.identifier.volume | 20 | - |
dc.identifier.issue | 1 | - |
dc.identifier.spage | 225 | - |
dc.identifier.epage | 234 | - |
dc.relation.doi | 10.1007/s10895-009-0542-3 | - |
dc.textversion | author | - |
dc.identifier.pmid | 19821013 | - |
dcterms.accessRights | open access | - |
dc.identifier.pissn | 1053-0509 | - |
dc.identifier.eissn | 1573-4994 | - |
出現コレクション: | 学術雑誌掲載論文等 |
このリポジトリに保管されているアイテムはすべて著作権により保護されています。