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タイトル: The universal mechanism for iron translocation to the ferroxidase site in ferritin, which is mediated by the well conserved transit site.
著者: Masuda, Taro  KAKEN_id
Goto, Fumiyuki
Yoshihara, Toshihiro
Mikami, Bunzo  kyouindb  KAKEN_id
著者名の別形: 増田, 太郎
キーワード: Ferritin
Ferroxidase site
Metal sequestration
Transit site
発行日: 10-Sep-2010
出版者: Elsevier Inc.
誌名: Biochemical and biophysical research communications
巻: 400
号: 1
開始ページ: 94
終了ページ: 99
抄録: Ferritins are ubiquitous iron storage proteins. Recently, we identified a novel metal-binding site, transit site, in the crystal structure of phytoferritin. To elucidate the function of the transit site in ferritin from other species, we prepared transit-site-deficient mutants of human H ferritin, E140A and E140Q, and their iron oxidation kinetics was analyzed. The initial velocities of iron oxidization were reduced in the variants, especially in E140Q. The crystal structure of E140Q showed that the side chain of the mutated Gln140 was fixed by a hydrogen bond, whereas that of native Glu140 was flexible. These results suggest that the conserved transit site also has a function to assist with the metal ion sequestration to the ferroxidase site in ferritins from vertebrates.
著作権等: © 2010 Elsevier Inc.
この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
This is not the published version. Please cite only the published version.
URI: http://hdl.handle.net/2433/128933
DOI(出版社版): 10.1016/j.bbrc.2010.08.017
PubMed ID: 20705053
出現コレクション:学術雑誌掲載論文等

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