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タイトル: piggyBac transposon-mediated long-term gene expression in mice.
著者: Nakanishi, Hideyuki
Higuchi, Yuriko  kyouindb  KAKEN_id
Kawakami, Shigeru
Yamashita, Fumiyoshi  kyouindb  KAKEN_id
Hashida, Mitsuru  kyouindb  KAKEN_id
著者名の別形: 中西, 秀之
橋田, 充
発行日: 26-Jan-2010
出版者: American Society of Gene & Cell Therapy
誌名: Molecular therapy
巻: 18
号: 4
開始ページ: 707
終了ページ: 714
抄録: Transposons are promising systems for somatic gene integration because they can not only integrate exogenous genes efficiently, but also be delivered to a variety of organs using a range of transfection methods. piggyBac (PB) transposon has a high transposability in mammalian cells in vitro, and has been used for genetic and preclinical studies. However, the transposability of PB in mammalian somatic cells in vivo has not been demonstrated yet. Here, we demonstrated PB-mediated sustained gene expression in adult mice. We constructed PB-based plasmid DNA (pDNA) containing reporter [firefly and Gaussia luciferase (Gluc)] genes. Mice were transfected by injection of these pDNAs using a hydrodynamics-based procedure, and the conditions for high-level sustained gene expression were examined. Consequently, gene expressions were sustained over 2 months. Our results suggest that PB is useful for organ-selective somatic integration and sustained gene expression in mammals, and will contribute to basic genetic studies and gene therapies.
著作権等: © 2010 American Society of Gene & Cell Therapy
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/129434
DOI(出版社版): 10.1038/mt.2009.302
PubMed ID: 20104210
出現コレクション:学術雑誌掲載論文等

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