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Title: piggyBac transposon-mediated long-term gene expression in mice.
Authors: Nakanishi, Hideyuki
Higuchi, Yuriko  kyouindb  KAKEN_id  orcid (unconfirmed)
Kawakami, Shigeru
Yamashita, Fumiyoshi  kyouindb  KAKEN_id  orcid (unconfirmed)
Hashida, Mitsuru  kyouindb  KAKEN_id
Author's alias: 中西, 秀之
橋田, 充
Issue Date: 26-Jan-2010
Publisher: American Society of Gene & Cell Therapy
Journal title: Molecular therapy
Volume: 18
Issue: 4
Start page: 707
End page: 714
Abstract: Transposons are promising systems for somatic gene integration because they can not only integrate exogenous genes efficiently, but also be delivered to a variety of organs using a range of transfection methods. piggyBac (PB) transposon has a high transposability in mammalian cells in vitro, and has been used for genetic and preclinical studies. However, the transposability of PB in mammalian somatic cells in vivo has not been demonstrated yet. Here, we demonstrated PB-mediated sustained gene expression in adult mice. We constructed PB-based plasmid DNA (pDNA) containing reporter [firefly and Gaussia luciferase (Gluc)] genes. Mice were transfected by injection of these pDNAs using a hydrodynamics-based procedure, and the conditions for high-level sustained gene expression were examined. Consequently, gene expressions were sustained over 2 months. Our results suggest that PB is useful for organ-selective somatic integration and sustained gene expression in mammals, and will contribute to basic genetic studies and gene therapies.
Rights: © 2010 American Society of Gene & Cell Therapy
This is not the published version. Please cite only the published version.
DOI(Published Version): 10.1038/mt.2009.302
PubMed ID: 20104210
Appears in Collections:Journal Articles

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