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j.biomaterials.2010.09.013.pdf | 633.35 kB | Adobe PDF | 見る/開く |
タイトル: | Biodegradable CpG DNA hydrogels for sustained delivery of doxorubicin and immunostimulatory signals in tumor-bearing mice. |
著者: | Nishikawa, Makiya Mizuno, Yumiko Mohri, Kohta Matsuoka, Nao Rattanakiat, Sakulrat Takahashi, Yuki https://orcid.org/0000-0002-8772-2772 (unconfirmed) Funabashi, Hisakage Luo, Dan Takakura, Yoshinobu https://orcid.org/0000-0002-7359-2647 (unconfirmed) |
著者名の別形: | 西川, 元也 |
キーワード: | DNA Drug delivery Hydrogel Immunostimulation Controlled drug release |
発行日: | 5-Oct-2010 |
出版者: | Elsevier BV |
誌名: | Biomaterials |
抄録: | Immunostimulatory CpG DNA was self-assembled to form DNA hydrogels for use as a sustained delivery system for both intercalated doxorubicin (DXR) and immunostimulatory CpG motifs for cancer treatment. X-shaped DNA (X-DNA) was designed as a building unit, and underwent ligation to form DNA hydrogels. Two types of X-DNA were constructed using four oligodeoxynucleotides each, one containing six potent CpG motifs (CpG X-DNA) and the other with none (CpG-free X-DNA). CpG X-DNA was more effective than its components or the CpG-free counterpart in terms of the production of tumor necrosis factor-α from murine macrophage-like RAW264.7 cells, as well as maturation of the murine dendritic DC2.4 cells. The cytotoxic effects of X-DNA, DXR and their complexes were examined in a co-culture system of colon26/Luc cells, a murine adenocarcinoma clone stably expressing firefly luciferase, and RAW264.7 cells. DXR/CpG X-DNA showed the highest ability to inhibit the proliferation of colon26/Luc cells. DXR was slowly released from CpG DNA hydrogels. Injections of DXR/CpG DNA hydrogels into a subcutaneous colon26 tumor effectively inhibited tumor growth. These results show that CpG DNA hydrogels are an effective sustained system for delivery of immunostimulatory signals to TLR9-positive immune cells and DXR to cancer cells. |
著作権等: | © 2010 Published by Elsevier Ltd. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/131741 |
DOI(出版社版): | 10.1016/j.biomaterials.2010.09.013 |
PubMed ID: | 20932569 |
出現コレクション: | 学術雑誌掲載論文等 |
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