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j.biomaterials.2010.09.013.pdf633.35 kBAdobe PDF見る/開く
タイトル: Biodegradable CpG DNA hydrogels for sustained delivery of doxorubicin and immunostimulatory signals in tumor-bearing mice.
著者: Nishikawa, Makiya  kyouindb  KAKEN_id
Mizuno, Yumiko
Mohri, Kohta
Matsuoka, Nao
Rattanakiat, Sakulrat
Takahashi, Yuki  kyouindb  KAKEN_id
Funabashi, Hisakage
Luo, Dan
Takakura, Yoshinobu  kyouindb  KAKEN_id
著者名の別形: 西川, 元也
キーワード: DNA
Drug delivery
Hydrogel
Immunostimulation
Controlled drug release
発行日: 5-Oct-2010
出版者: Elsevier Ltd.
誌名: Biomaterials
抄録: Immunostimulatory CpG DNA was self-assembled to form DNA hydrogels for use as a sustained delivery system for both intercalated doxorubicin (DXR) and immunostimulatory CpG motifs for cancer treatment. X-shaped DNA (X-DNA) was designed as a building unit, and underwent ligation to form DNA hydrogels. Two types of X-DNA were constructed using four oligodeoxynucleotides each, one containing six potent CpG motifs (CpG X-DNA) and the other with none (CpG-free X-DNA). CpG X-DNA was more effective than its components or the CpG-free counterpart in terms of the production of tumor necrosis factor-α from murine macrophage-like RAW264.7 cells, as well as maturation of the murine dendritic DC2.4 cells. The cytotoxic effects of X-DNA, DXR and their complexes were examined in a co-culture system of colon26/Luc cells, a murine adenocarcinoma clone stably expressing firefly luciferase, and RAW264.7 cells. DXR/CpG X-DNA showed the highest ability to inhibit the proliferation of colon26/Luc cells. DXR was slowly released from CpG DNA hydrogels. Injections of DXR/CpG DNA hydrogels into a subcutaneous colon26 tumor effectively inhibited tumor growth. These results show that CpG DNA hydrogels are an effective sustained system for delivery of immunostimulatory signals to TLR9-positive immune cells and DXR to cancer cells.
著作権等: © 2010 Published by Elsevier Ltd.
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/131741
DOI(出版社版): 10.1016/j.biomaterials.2010.09.013
PubMed ID: 20932569
出現コレクション:学術雑誌掲載論文等

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