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j.biomaterials.2010.09.013.pdf633.35 kBAdobe PDF見る/開く
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dc.contributor.authorNishikawa, Makiyaja
dc.contributor.authorMizuno, Yumikoja
dc.contributor.authorMohri, Kohtaja
dc.contributor.authorMatsuoka, Naoja
dc.contributor.authorRattanakiat, Sakulratja
dc.contributor.authorTakahashi, Yukija
dc.contributor.authorFunabashi, Hisakageja
dc.contributor.authorLuo, Danja
dc.contributor.authorTakakura, Yoshinobuja
dc.contributor.alternative西川, 元也ja
dc.date.accessioned2010-11-16T01:19:02Z-
dc.date.available2010-11-16T01:19:02Z-
dc.date.issued2010-10-05ja
dc.identifier.issn0142-9612ja
dc.identifier.urihttp://hdl.handle.net/2433/131741-
dc.description.abstractImmunostimulatory CpG DNA was self-assembled to form DNA hydrogels for use as a sustained delivery system for both intercalated doxorubicin (DXR) and immunostimulatory CpG motifs for cancer treatment. X-shaped DNA (X-DNA) was designed as a building unit, and underwent ligation to form DNA hydrogels. Two types of X-DNA were constructed using four oligodeoxynucleotides each, one containing six potent CpG motifs (CpG X-DNA) and the other with none (CpG-free X-DNA). CpG X-DNA was more effective than its components or the CpG-free counterpart in terms of the production of tumor necrosis factor-α from murine macrophage-like RAW264.7 cells, as well as maturation of the murine dendritic DC2.4 cells. The cytotoxic effects of X-DNA, DXR and their complexes were examined in a co-culture system of colon26/Luc cells, a murine adenocarcinoma clone stably expressing firefly luciferase, and RAW264.7 cells. DXR/CpG X-DNA showed the highest ability to inhibit the proliferation of colon26/Luc cells. DXR was slowly released from CpG DNA hydrogels. Injections of DXR/CpG DNA hydrogels into a subcutaneous colon26 tumor effectively inhibited tumor growth. These results show that CpG DNA hydrogels are an effective sustained system for delivery of immunostimulatory signals to TLR9-positive immune cells and DXR to cancer cells.ja
dc.format.mimetypeapplication/pdfja
dc.language.isoengja
dc.publisherElsevier Ltd.ja
dc.rights© 2010 Published by Elsevier Ltd.ja
dc.rightsThis is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。ja
dc.subjectDNAja
dc.subjectDrug deliveryja
dc.subjectHydrogelja
dc.subjectImmunostimulationja
dc.subjectControlled drug releaseja
dc.titleBiodegradable CpG DNA hydrogels for sustained delivery of doxorubicin and immunostimulatory signals in tumor-bearing mice.ja
dc.type.niitypeJournal Articleja
dc.identifier.ncidAA00110092ja
dc.identifier.jtitleBiomaterialsja
dc.relation.doi10.1016/j.biomaterials.2010.09.013ja
dc.textversionauthorja
dc.identifier.pmid20932569ja
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