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| S1744309110029040.pdf | 112.37 kB | Adobe PDF | View/Open |
| Title: | Crystallization and preliminary X-ray analysis of the major peanut allergen Ara h 1 core region. |
| Authors: | Cabanos, Cerrone    https://orcid.org/0000-0001-5915-3683 (unconfirmed)Urabe, Hiroyuki Masuda, Taro Tandang-Silvas, Mary Rose Utsumi, Shigeru Mikami, Bunzo Maruyama, Nobuyuki https://orcid.org/0000-0002-1369-9629 (unconfirmed) |
| Author's alias: | 丸山, 伸之 |
| Keywords: | peanut allergens Ara h 1 |
| Issue Date: | 1-Sep-2010 |
| Publisher: | International Union of Crystallography |
| Journal title: | Acta crystallographica. Section F, Structural biology and crystallization communications |
| Volume: | 66 |
| Issue: | Pt 9 |
| Start page: | 1071 |
| End page: | 1073 |
| Abstract: | Peanuts contain some of the most potent food allergens known to date. Ara h 1 is one of the three major peanut allergens. As a first step towards three-dimensional structure elucidation, recombinant Ara h 1 core region was cloned, expressed in Escherichia coli and purified to homogeneity. Crystals were obtained using 0.1 M sodium citrate pH 5.6, 0.1 M NaCl, 15% PEG 400 as precipitant. The crystals diffracted to 2.25 A resolution using synchrotron radiation and belonged to the monoclinic space group C2, with unit-cell parameters a=156.521, b=88.991, c=158.971 A, beta=107.144 degrees. Data were collected at the BL-38B1 station of SPring-8 (Hyogo, Japan). |
| Rights: | © International Union of Crystallography |
| URI: | http://hdl.handle.net/2433/131796 |
| DOI(Published Version): | 10.1107/S1744309110029040 |
| PubMed ID: | 20823529 |
| Appears in Collections: | Journal Articles |
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