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タイトル: | Crystallization and preliminary X-ray analysis of the major peanut allergen Ara h 1 core region. |
著者: | Cabanos, Cerrone https://orcid.org/0000-0001-5915-3683 (unconfirmed) Urabe, Hiroyuki Masuda, Taro Tandang-Silvas, Mary Rose Utsumi, Shigeru Mikami, Bunzo Maruyama, Nobuyuki https://orcid.org/0000-0002-1369-9629 (unconfirmed) |
著者名の別形: | 丸山, 伸之 |
キーワード: | peanut allergens Ara h 1 |
発行日: | 1-Sep-2010 |
出版者: | International Union of Crystallography |
誌名: | Acta crystallographica. Section F, Structural biology and crystallization communications |
巻: | 66 |
号: | Pt 9 |
開始ページ: | 1071 |
終了ページ: | 1073 |
抄録: | Peanuts contain some of the most potent food allergens known to date. Ara h 1 is one of the three major peanut allergens. As a first step towards three-dimensional structure elucidation, recombinant Ara h 1 core region was cloned, expressed in Escherichia coli and purified to homogeneity. Crystals were obtained using 0.1 M sodium citrate pH 5.6, 0.1 M NaCl, 15% PEG 400 as precipitant. The crystals diffracted to 2.25 A resolution using synchrotron radiation and belonged to the monoclinic space group C2, with unit-cell parameters a=156.521, b=88.991, c=158.971 A, beta=107.144 degrees. Data were collected at the BL-38B1 station of SPring-8 (Hyogo, Japan). |
著作権等: | © International Union of Crystallography |
URI: | http://hdl.handle.net/2433/131796 |
DOI(出版社版): | 10.1107/S1744309110029040 |
PubMed ID: | 20823529 |
出現コレクション: | 学術雑誌掲載論文等 |
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