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Title: | Crystallization and preliminary X-ray analysis of the major peanut allergen Ara h 1 core region. |
Authors: | Cabanos, Cerrone ![]() ![]() ![]() Urabe, Hiroyuki Masuda, Taro ![]() ![]() Tandang-Silvas, Mary Rose Utsumi, Shigeru Mikami, Bunzo ![]() ![]() Maruyama, Nobuyuki ![]() ![]() |
Author's alias: | 丸山, 伸之 |
Keywords: | peanut allergens Ara h 1 |
Issue Date: | 1-Sep-2010 |
Publisher: | International Union of Crystallography |
Journal title: | Acta crystallographica. Section F, Structural biology and crystallization communications |
Volume: | 66 |
Issue: | Pt 9 |
Start page: | 1071 |
End page: | 1073 |
Abstract: | Peanuts contain some of the most potent food allergens known to date. Ara h 1 is one of the three major peanut allergens. As a first step towards three-dimensional structure elucidation, recombinant Ara h 1 core region was cloned, expressed in Escherichia coli and purified to homogeneity. Crystals were obtained using 0.1 M sodium citrate pH 5.6, 0.1 M NaCl, 15% PEG 400 as precipitant. The crystals diffracted to 2.25 A resolution using synchrotron radiation and belonged to the monoclinic space group C2, with unit-cell parameters a=156.521, b=88.991, c=158.971 A, beta=107.144 degrees. Data were collected at the BL-38B1 station of SPring-8 (Hyogo, Japan). |
Rights: | © International Union of Crystallography |
URI: | http://hdl.handle.net/2433/131796 |
DOI(Published Version): | 10.1107/S1744309110029040 |
PubMed ID: | 20823529 |
Appears in Collections: | Journal Articles |

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