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タイトル: | Identification of serum proteins that bind with S100A8, S100A9 and S100A8/A9: clinical significance of using proteins for monitoring the postoperative condition of liver recipients. |
著者: | Namura, Tomoyo Arai, Satoshi Okawa, Katsuya Koike, Akiko Yamada, Sachiko Saita, Naoko Nagae, Akiko Itoh, Hiroshi Totani, Masayuki Uemoto, Shinji Ikemoto, Masaki |
著者名の別形: | 南村, 知代 池本, 正生 |
キーワード: | S100 proteins Fibronectin Inflammation Neutrophil Macrophage CRP |
発行日: | 11-Nov-2010 |
出版者: | Elsevier B.V. |
誌名: | Clinica chimica acta; international journal of clinical chemistry |
巻: | 411 |
号: | 21-22 |
開始ページ: | 1766 |
終了ページ: | 1773 |
抄録: | BACKGROUND: Serum proteins that non-specifically bind with human S100A8/A9 (h-S100A8/A9) have been proposed. Our aim was to isolate and identify these proteins, and verify their clinical significance for monitoring the postoperative condition of liver recipients, and further to discuss the transportation of human fibronectin (h-FN) with h-S100A8/A9 and its functional role in vivo. METHODS: To isolate the serum proteins, recombinant human S100A8, S100A9 and S100A8/A9 affinity columns were used. Proteins were identified by mass spectrometry. Two enzyme-linked immunosorbent assays (ELISA) were used to measure h-S100A8/A9 and h-FN in the sera of liver recipients. Flow cytometry was employed to detect h-S100A8/A9 and h-FN on immunological cells. Western blotting was used to confirm serum constituents using antibodies specific to each constituent. RESULTS: One of the proteins was identified with h-FN, and its fluctuation pattern in the serum of the recipient was in contrast to that of CRP. Flow cytometry showed a positive reaction for h-S100A8/A9 and h-FN on neutrophils and monocytes, indicating that both proteins exist on these cells. CONCLUSIONS: The h-FN could be transported with S100A8/A9 in blood and/or on immunological cells, and effectively prevent further attack by various internal oxidants or repair damaged liver tissue in vivo. |
著作権等: | © 2010 Elsevier B.V. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/131814 |
DOI(出版社版): | 10.1016/j.cca.2010.07.029 |
PubMed ID: | 20674560 |
出現コレクション: | 学術雑誌掲載論文等 |
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