ダウンロード数: 536
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
j.metabol.2010.03.009.pdf | 1.57 MB | Adobe PDF | 見る/開く |
タイトル: | Berberine-induced activation of 5'-adenosine monophosphate-activated protein kinase and glucose transport in rat skeletal muscles. |
著者: | Ma, Xiao Egawa, Tatsuro https://orcid.org/0000-0001-9363-1589 (unconfirmed) Kimura, Hajime Karaike, Kouhei Masuda, Shinya Iwanaka, Nobumasa Hayashi, Tatsuya https://orcid.org/0000-0001-7600-4735 (unconfirmed) |
著者名の別形: | 林, 達也 |
発行日: | Nov-2010 |
出版者: | Elsevier Inc. |
誌名: | Metabolism: clinical and experimental |
巻: | 59 |
号: | 11 |
開始ページ: | 1619 |
終了ページ: | 1627 |
抄録: | Berberine (BBR) is the main alkaloid of Coptis chinensis, which has been used as a folk medicine to treat diabetes mellitus in Asian countries. We explored the possibility that 5'-adenosine monophosphate-activated protein kinase (AMPK) is involved in metabolic enhancement by BBR in skeletal muscle, the important tissue for glucose metabolism. Isolated rat epitrochlearis and soleus muscles were incubated in a buffer containing BBR, and activation of AMPK and related events were examined. In response to BBR treatment, the Thr(172) phosphorylation of the catalytic α-subunit of AMPK, an essential step for full kinase activation, increased in a dose- and time-dependent manner. Ser(79) phosphorylation of acetyl-coenzyme A carboxylase, an intracellular substrate of AMPK, increased correspondingly. Analysis of isoform-specific AMPK activity revealed that BBR activated both the α1 and α2 isoforms of the catalytic subunit. This increase in enzyme activity was associated with an increased rate of 3-O-methyl-d-glucose transport in the absence of insulin and with phosphorylation of AS160, a signaling intermediary leading to glucose transporter 4 translocation. The intracellular energy status estimated from the phosphocreatine concentration was decreased by BBR. These results suggest that BBR acutely stimulates both AMPKα1 and AMPKα2 and insulin-independent glucose transport in skeletal muscle with a reduction of the intracellular energy status. |
著作権等: | © 2010 Elsevier Inc. This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/131860 |
DOI(出版社版): | 10.1016/j.metabol.2010.03.009 |
PubMed ID: | 20423742 |
出現コレクション: | 学術雑誌掲載論文等 |
このリポジトリに保管されているアイテムはすべて著作権により保護されています。