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Title: Kinetics of conformational changes of the FKF1-LOV domain upon photoexcitation.
Authors: Nakasone, Yusuke  kyouindb  KAKEN_id
Zikihara, Kazunori
Tokutomi, Satoru
Terazima, Masahide  kyouindb  KAKEN_id  orcid (unconfirmed)
Author's alias: 寺嶋, 正秀
Issue Date: 1-Dec-2010
Publisher: Elsevier Inc.
Journal title: Biophysical journal
Volume: 99
Issue: 11
Start page: 3831
End page: 3839
Abstract: The photochemical reaction dynamics of a light-oxygen-voltage (LOV) domain from the blue light sensor protein, FKF1 (flavin-binding Kelch repeat F-box) was studied by means of the pulsed laser-induced transient grating method. The observed absorption spectral changes upon photoexcitation were similar to the spectral changes observed for typical LOV domain proteins (e.g., phototropins). The adduct formation took place with a time constant of 6 μs. After this reaction, a significant conformational change with a time constant of 6 ms was observed as a change in the diffusion coefficient. An FKF1-LOV mutant without the conserved loop connecting helices E and F, which is present only in the FKF1/LOV Kelch protein 2/ZEITLUPE family, did not show these slow phase dynamics. This result indicates that the conformational change in the loop region represents a major change in the FKF1-LOV photoreaction.
Rights: © 2010 Biophysical Society Published by Elsevier Inc.
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
DOI(Published Version): 10.1016/j.bpj.2010.10.005
PubMed ID: 21112308
Appears in Collections:Journal Articles

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